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            • ELISA Kits ›
            • DPP4 ELISA Kits

            Invitrogen

            Mouse DPPIV (DPP4/CD26) ELISA Kit

            1 Reference
            View all (3) DPP4 ELISA Kits
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              Mouse DPPIV (DPP4/CD26) ELISA Kit (EMDPP4)
              Group 53 Created with Sketch.

              FIGURE: 1 / 2

              Mouse DPPIV (DPP4/CD26) ELISA Kit

              Sandwich ELISA Kit
              View Product

              Mouse DPPIV (DPP4/CD26) ELISA Kit (EMDPP4)
              videoThumbnail
              ►

              Product Specifications

              Analytical sensitivity

              0.45 ng/mL

              Assay range

              0.41-100 ng/mL

              Sample type/volume

              Plasma
              5 µL
              Serum
              5 µL
              Supernatant
              100 µL

              Hands-on time

              1 hr 20 min

              Time-to-result

              4 hr 45 min

              Homogenous (no wash)

              No

              Interassay CV

              <12%

              Intraassay CV

              <10%

              Instrument

              Absorbance Microplate Reader

              Product size

              96 Tests

              Contents

              Pre-coated 96 well plate
              Standard
              Assay Diluent concentrate
              Biotinylated Detection Antibody
              SAV-HRP
              Wash Buffer
              Chromogen
              Stop Solution
              Adhesive Plate Covers

              Shipping conditions

              Wet ice

              Storage

              2-8°C

              Protein name

              DPP4

              Protein family

              Proteases Peptidases & Inhibitors

              Species (tested)

              Mouse

              Assay kit format

              Sandwich ELISA Kit

              Detector antibody conjugate

              Biotin

              Label or dye

              HRP

              About This Kit

              The Mouse DPPIV (DPP4/CD26) ELISA quantitates Ms DPPIV in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms DPPIV.

              Principle of the method

              The Mouse DPPIV solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

              Rigorous validation

              Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

              Target information

              CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family. CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity. CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.

              For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

              Bioinformatics

              Gene aliases : Cd26, Dpp-4, Dpp4, THAM

              Gene ID : (Mouse) 13482

              Gene symbol : Dpp4

              Protein Aliases : CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP-IV, T-cell activation antigen CD26, THAM, Thymocyte-activating molecule

              UniProt ID (Mouse) P28843

              Recommended Products

              IL-6 Human Matched Antibody Pair

              Because you are interested in IL-6 Human ELISA Kit.

              SIZE

              10 x 96 tests

              PRICE

              USD 400.00

              Cat # CHC1263

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              If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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