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Multispecies MAPK Family Activation InstantOne™ ELISA Kit
Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
JNK1/JNK2/JNK3/ERK1/2/p38 MAPK alpha
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), p38 MAPK (human, mouse), and JNK 1/2 (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
MAP kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. MAPKBP-1 (mitogen-activated protein kinase binding protein 1), also known as JNKBP-1, is a 1,514 amino acid protein that contains twelve WD repeats. Induced by TRAF2 (TNF receptor-associated factor 2) and Tak1 (TGF-β-activated kinase 1), MAPKBP-1 is thought to act an adaptor protein for NFκB (nuclear factor κ-B) activation. MAPKBP-1 interacts with JNK3 and may promote TRAF2 polyubiquitination. MAPKBP-1 exists as six alternatively spliced variants and is encoded by a gene located on human chromosome 15. Human chromosome 15 houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CSBP, CSBP1, CSBP2, CSPB1, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, EXIP, HS44KDAP, HUMKER1A, MAPK1, MAPK14, MAPK2, MAPK3, MXI2, p38, p38ALPHA, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM1, PRKM14, PRKM15, PRKM2, PRKM3, RK, SAPK2A
Gene ID : (Human) 5594, (Human) 1432, (Human) 5595
Gene symbol : MAPK1, MAPK14, MAPK3
Protein Aliases : CSAID-binding protein, Csaids binding protein, cytokine suppressive anti-inflammatory drug binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 14, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 1, MAPK 2, MAX-interacting protein 2, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, Mitogen-activated protein kinase p38 alpha, p38 MAP kinase, p38 mitogen activated protein kinase, p38alpha Exip, p42-MAPK, p44-ERK1, p44-MAPK, protein tyrosine kinase ERK2, SAPK2a, Stress-activated protein kinase 2a
UniProt ID (Human) P28482, (Human) Q16539, (Human) P27361
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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