Molecular Biology Literature
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Molecular biology |  Cloning |  PCR |  Reverse transcription |  Electrophoresis
Alternative to AMV-based reverse transcriptases

Invitrogen SuperScript IV Reverse Transcriptase performance comparison to AMV-based enzymes

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Amplification robustness of thermal cyclers

Thermal cycler amplification robustness: a comparison of several models

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Bacterial 16S rRNA gene detection

Bacterial DNA detection in samples with low amounts of microbial genomic DNA, using degenerate primers

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Blunt-end cloning strategies

The Invitrogen Anza Restriction Enzyme Cloning System provides multiple options for blunt-end cloning

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Colony PCR

Direct PCR from bacterial, yeast, and mammalian cells using Platinum SuperFi II DNA Polymerase

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Comparison of amplification uniformity across thermal cyclers

Thermal cycler sample amplification uniformity: a comparison of several models

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Comparison of error rates in synthetic genes

Evaluation of linear synthetic DNA fragments from separate suppliers

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Comparison of sample evaporation across thermal cyclers

Sample evaporation: A comparison of several thermal cycler models

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Dephosphorylation protocols

Optimal dephosphorylation protocols using Invitrogen Anza Alkaline Phosphatase

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Direct cDNA synthesis

SuperScript IV CellsDirect cDNA Synthesis Kit: A more sensitive, reliable, and flexible kit for direct cDNA synthesis

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Direct detection of target DNA in cell samples

Real-time PCR using Platinum Direct PCR Universal Master Mix

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Direct PCR from blood using a high-fidelity enzyme

Direct PCR from blood using Platinum SuperFi II DNA Polymerase

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Direct PCR from blood using a hot-start enzyme

Direct PCR from blood using Platinum II Taq Hot-Start DNA Polymerase

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Directional cloning strategies

The Anza Restriction Enzyme Cloning System simplifies directional cloning

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DNA ladder stability

DNA ladders shipment at ambient temperature

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DNA-free enzymes

Single-use technology in production of DNA-free PCR reagents

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Enabling amplification of AT-rich DNA sequences

Platinum II Taq Hot-Start DNA Polymerase can amplify AT-rich DNA sequences with minor optimization

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Enhanced protein expression by gene optimization

Essential strategies to optimize protein expression

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Gibson Assembly cloning technologies

Evaluation of GeneArt Gibson Assembly EX Cloning technology to build large and complex assemblies

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Gibson Assembly cloning utilizing homology and oligonucleotide stiching techniques

Utilizing both homology and oligonucleotide stitching techniques to build large constructs

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High-throughput PCR

Improving PCR lab throughput by fast co-cycling of several assays in the same run

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How-to guide for PCR plastics

How to use Applied Biosystems MicroAmp reaction plates, tube strips, and tubes

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Improved technology to gradient blocks

Applied Biosystems VeriFlex temperature control technology for thermal cycling

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Ligation considerations

Generating recombinant DNA clones with Invitrogen Anza T4 DNA Ligase Master Mix

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Metagenomic study of bacteria in lake water

Direct amplification of 16S rRNA gene sequences, followed by next-generation sequencing of amplicons and data analysis

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Mouse genotyping

Fast and convenient PCR with DNA extracted from mouse tail by alkaline lysis

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Multiplex PCR with a high-fidelity enzyme

Multiplex PCR with Platinum SuperFi II DNA Polymerase

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Multiplex PCR with a hot-start enzyme

Multiplex PCR with Platinum II Taq Hot-start DNA Polymerase

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Nucleic acid stains

Invitrogen SYBR nucleic acid gel stains

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One-step RT-PCR

Advanced analysis with the SuperScript IV One-Step RT-PCR System

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PCR plate selection and amplification uniformity

Assessment of PCR plate performance in the Automated Thermal Cycler

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Restriction digestion

Restriction enzyme isoschizomers and key considerations

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Reverse transcriptase’s attributes

Invitrogen SuperScript IV Reverse Transcriptase

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RT-qPCR master mix

Invitrogen SuperScript IV VILO Master Mix for optimal RT-qPCR

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RT-qPCR with blood samples

Reliable and sensitive RT-qPCR analysis of whole-blood RNA samples

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RT-qPCR with plant samples

Improved RT-qPCR analysis of difficult plant RNA samples

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Safer nucleic acid stain

SYBR Safe DNA Gel Stain: assessment of mutagenicity and environmental safety

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SARS-CoV-2 cDNA synthesis

SuperScript IV reverse transcription protocols using SARS-CoV-2 research samples

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Simple and fast genotyping workflow

A fast, high-throughput genotyping protocol for plants and mice using direct PCR and E-Gel electrophoresis.

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Site-directed mutagenesis

Fast and efficient site-directed mutagenesis with Platinum SuperFi II DNA Polymerase

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SNP detection by ARMS PCR

Detection of SNPs by fast, simple, and economical PCR using Platinum II Taq Hot-Start DNA Polymerase

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Successful amplification of AT-rich DNA sequences

Efficient amplification of AT-rich DNA sequences using Platinum SuperFi II DNA Polymerase

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Technical features of a thermal cycler

VeritiPro Thermal Cycler—technical comparison to the Veriti Thermal Cycler

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Temperature accuracy of thermal cyclers

Thermal cycler temperature accuracy: a comparison of several models

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Thermal cycler reliability

Reliability and quality testing of our thermal cyclers

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Thermal cycling and ramp rates

Thermal cyclers: key thermal cycling concepts and ramp rates

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Type IIs cloning (Golden Gate assembly)

Cloning with Anza type IIs restriction endonucleases

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Resources

Molecular biology

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