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Murashige and Skoog Complete Medium-50X Concentrate (1) contains Murashige and Skoog salts sucrose and vitamins. This medium is easy to prepare and can be used for most plant tissue culture applications.
Catalog Number(s)
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Vitamins | |||
| Myo-Inositol | 180.0 | 100.0 | 0.5555556 |
| Nicotinic acid (Niacin) | 123.0 | 0.05 | 4.0650408E-4 |
| Pyridoxine hydrochloride | 206.0 | 0.05 | 2.4271845E-4 |
| Thiamine hydrochloride | 337.0 | 0.1 | 2.967359E-4 |
| Inorganic Salts | |||
| Ammonium Nitrate (NH4NO3) | 80.0 | 1650.0 | 20.625 |
| Boric Acid (H3BO3) | 62.0 | 6.2 | 0.099999994 |
| Calcium Chloride (CaCl2) (anhyd.) | 111.0 | 332.2 | 2.9927928 |
| Cobalt Chloride (CoCl2-6H2O) | 238.0 | 0.025 | 1.0504202E-4 |
| Cupric Sulfate (CuSO4-6H20) | 250.0 | 0.025 | 1.00000005E-4 |
| Ferric sulfate (FeSO4-7H2O) | 278.0 | 27.8 | 0.099999994 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120.0 | 180.7 | 1.5058333 |
| Manganese Sulfate (MnSO4-H20) | 169.0 | 16.9 | 0.099999994 |
| Potassium Iodide (KI) | 166.0 | 0.83 | 0.005 |
| Potassium Nitrate (KNO3) | 101.0 | 1900.0 | 18.811882 |
| Potassium Phosphate monobasic (KH2PO4) | 136.0 | 170.0 | 1.25 |
| Sodium Molybdate (Na2MoO4-2H20) | 243.0 | 0.25 | 0.0010288066 |
| Zinc sulfate (ZnSO4-7H2O) | 288.0 | 8.6 | 0.029861113 |
| Other Components | |||
| Glycine (Free Base) | 75.0 | 2.0 | 0.026666667 |
| MES | 213.0 | 500.0 | 2.3474178 |
| Sodium EDTA (Na2-EDTA) | 372.0 | 37.26 | 0.100161284 |
| Sucrose | 342.0 | 30000.0 | 87.7193 |
1. Murashige, T. and Skoog, F. (1962) Physiol. Plant. 15, 485.