Dynamic binding capacity at 10% breakthrough at different residence times

High dynamic binding capacities (DBCs) are obtained under a wide range of residence times, especially at lower residence times and higher linear flow rates with POROS™ MabCapture™ A Select. POROS MabCapture A Select shows higher DBCs across all residence times when compared to Pierce™ Protein A PLUS Agarose. Experimental conditions: Column area: 0.5 cmD x 5 cmL, Binding Buffer: 100 mM phosphate, 150 mM sodium chloride, pH 7.2.  Load conditions: 1 mg/mL IgG, Flow Rate: 500 cm/hr, 300 cm/hr, 150 cm/hr, 75 cm/hr, and 37.5 cm/hr.

High dynamic binding capacities (DBCs) are obtained under a wide range of residence times, especially at lower residence times and higher linear flow rates with POROS™ MabCapture™ A Select. POROS MabCapture A Select shows higher DBCs across all residence times when compared to Pierce™ Protein A PLUS Agarose. Experimental conditions: Column area: 0.5 cmD x 5 cmL, Binding Buffer: 100 mM phosphate, 150 mM sodium chloride, pH 7.2.  Load conditions: 1 mg/mL IgG, Flow Rate: 500 cm/hr, 300 cm/hr, 150 cm/hr, 75 cm/hr, and 37.5 cm/hr.

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