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Leading the future of flow cytometry and innovative solutions: please visit us at booth #301
Discover. Connect. Innovate.
Join us at CYTO 2025 to explore innovations in flow cytometry, imaging, and cell sorting. Whether you're looking to solve specific research challenges, streamline workflows, or experience the power of our latest technologies firsthand, this is your chance to engage directly with Thermo Fisher Scientific specialists.
Book your free one-on-one specialist session
Limited spots available
Take your research further with a personalized consultation or live demo. Whether you’re navigating complex challenges, optimizing your flow cytometry panel design, or exploring the latest in spectral technology, we’ll connect you with the right specialist—R&D scientists, software developers, hardware engineers, or panel design specialists.
Sessions are just 30 minutes—quick, impactful, and tailored to you.
Spots are limited and filling fast—reserve yours today!
- Tutorial session
- Posters
- New innovations
Don’t miss our tutorial sessions
Tuesday, June 3 | 12:15–1:15 PM | Mile High Ballroom 1A-C
Enhancing Cell Sorting with Imaging-Enabled Cytometry
Speakers: Nate Trujillo, PhD & Mike Ward, PhD
Abstract
The integration of advanced imaging cytometry with traditional cell sorting techniques offers a transformative approach to cellular analysis and sorting. In this presentation, we explore the synergistic use of the Invitrogen Bigfoot Spectral Cell Sorter and the Invitrogen Attune CytPix Flow Cytometer, an imaging-enabled flow cytometer, to enhance the precision and reliability of cell sorting processes. By leveraging the high-resolution imaging capabilities and additional morphological parameters of the Attune CytPix Flow Cytometer, a pre-sort assessment can be performed to evaluate cell quality, refine gating strategies, and help ensure proper populations are sorted to improve overall efficiency and accuracy. After sorting, the Attune CytPix platform can be used to verify the purity and composition of the sorted cell populations. This step is essential for verifying the success of the sorting protocol and helping ensure that the intended cell subset has been accurately isolated.
This study also investigated employment of the Attune CytPix platform to perform automated morphology analysis of acute myeloid leukemia (AML) samples. With a new, exceptional machine learning model, the Attune CytPix Flow Cytometer brings the benefits of brightfield imaging to introduce new morphological parameters and high dimensional tools such as uMAP for added data visualization and analysis. Using this technology, a cell is selected, and the software automatically searches for similar cells. From this data, we found that less than 1% of the sample showed cell binding between hyperdiploid leukemia cells and white blood cells. This methodology facilitates the detection of rare hyperdiploid cells, and their interactions with white blood cells—a phenomenon that reduces detection of leukemia cells and thwarts therapeutic efforts. The ability to discern different AML phenotypes based on morphology can open new avenues for research and therapeutic interventions, helping advance the field of leukemia research and treatment.
Wednesday, June 4 | 12:15–1:15 PM | Mile High Ballroom 1D
Boosting Core Facility Efficiency with Bigfoot & Attune Xenith
Speakers: Rachael Walker, PhD & William Kang
Abstract
Flow cytometry core facilities play a pivotal role in advancing research, requiring state-of-the-art tools to meet the demands of high-throughput and sometimes complex analyses. This talk will focus on the transformative impact of the Thermo Fisher Bigfoot Cell Sorter and Attune Xenith Flow Cytometer within these facilities. The Thermo Fisher Bigfoot Cell Sorter is a game-changer, offering exceptional sorting speed and precision, which significantly reduces processing times and increases sample throughput. The Attune Xenith Flow Cytometer, with its advanced spectral flow cytometry capabilities and acoustic focusing, allows for the simultaneous detection of multiple fluorochromes with high resolution and accuracy. These instruments not only enhance the analytical capabilities of core facilities but also provide substantial time savings, enabling more efficient workflow management and resource utilization. By integrating the Bigfoot Cell Sorter and Attune Xenith Flow Cytometer, at Babraham Institute, we have been able to achieve new levels of performance and productivity, ultimately driving forward scientific discovery and innovation.
The Invitrogen EVOS S1000 Spatial Imaging System is only available in participating countries and regions including US, Canada, Australia and Europe in 2025.
For Research Use Only. Not for use in diagnostic procedures.