Search Thermo Fisher Scientific
Cellular hydrogen peroxide detection in U2-OS cells using Premo™ Cellular Hydrogen Peroxide Sensor
U2-OS cells were plated on 35 mm dishes (MatTek) at a density of 75,000 cells and transduced with Premo™ Cellular Hydrogen Peroxide Sensor. After 48 hrs, time lapse imaging was done on a confocal microscope using 400 nm and 488 nm lasers for excitation with the emission at 515 nm. The images were taken every 15 seconds after addition of 25 µM hydrogen peroxide for 10 minutes.
