Cryo-EM University: Objective Lens, Stage, and Projection

Objective lens, focus, and eucentric height

23:10

The Objective Lens

Theoretical overview of the function of the objective lens in an electron microscope.
20:29

Focus

Explanation and review of when a lens is in focus.
6:54

Eucenctric Height

The importance of eucentric height.
17:42

How to set Eucentric Height

Demonstration of how to set the Eucentric height in the TEM User Interface.
49:10

Different Ways to Determine Focus

Theoretical explanation on the different ways to focus.
32:29

How to Focus on an Electron Microscope

Demonstration on how to focus the electron microscope in the TEM User Interface.

Projection

43:58

Projection and Magnification

Explanation of what is magnification and projection in an electron microscope.
24:40

Changing Magnification on an Electron Microscope

Demonstration on how to change magnification on an electron microscope through the TEM User Interface.
2:44

Camera Length

Demonstration of the changing camera length to change the magnification.

Effect of not being parallel on the image

11:09

Consequences of Not being Parallel

Overview and explanation of the consequences of when you are not parallel illuminating your specimen.
9:23

Imaging while Not being Parallel

Demonstration of the consequences of not parallel illuminating your specimen in the TEM User Interface.

Apertures and astigmatism

25:27

The Objective Aperture

Overview of the objective aperture and its function.
6:33

How to Align the Objective Aperture

Demonstration on how to align the objective aperture in the TEM User Interface.
6:04

Contrast Enhancement using the Objective Aperture

Demonstration of the contrast enhancement using the objective aperture in the TEM User Interface.
10:53

Objective Astigmatism

Overview and explanation of the objective lens astigmatism.
9:03

How to Correct Objective Astigmatism

Demonstration on how to set objective stigmation through the TEM User Interface.
11:01

Diffraction Astigmatism

Theoretical explanation on diffraction astigmatism, which comes from the diffraction lens.
3:24

When to Correct Diffraction Astigmatism

Demonstration of when to correct diffraction astigmatism in the TEM User Interface.

For Research Use Only. Not for use in diagnostic procedures.

1x1 image pixel for data collection