Quantitative proteomics strategies using Tandem Mass Tags (TMT) enable precise measurement of protein abundance from multiple samples into a single high-resolution LC/MS analysis. Increasingly, various biological experiments demand higher multiplexing and sample throughput with accurate quantitation. This webinar presents strategies that combine next generation technologies in multiplexing chemistry, hardware and software for increased quantitative performance and throughput efficiency on Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer. This includes Advanced SPS-MS3 approaches using next generation isobaric tags and real-time search. Together, these advances allow for intelligent acquisition methods that improve quantitation accuracy, precision and proteome depth.

About the presenter

Steven Gygi, Professor, Harvard University

Steven Gygi, Professor, Harvard University

Dr. Gygi received his Ph.D. from the University of Utah in the area of Pharmacology and Toxicology. He joined the faculty at Harvard Medical School in 2000. He is currently a Professor in the Department of Cell Biology. Research in Dr. Gygi’s lab centers around developing technologies and computational techniques for rapid, sensitive, quantitative and multiplexed analysis of protein expression profiles from diverse cellular settings.

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