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Feline genetics plays a vital role in research and veterinary care, providing essential insights into breed identification and trait prediction that enhance our understanding of feline health and well-being. Due to their high-throughput and unparalleled genomic coverage, high-density microarrays are widely used for SNP genotyping. With these arrays, researchers can test thousands of samples across tens of thousands of SNPs simultaneously, in a simple high-throughput workflow. In our effort to develop an Axiom high-density microarray, over 2,000,000 markers were screened across over 2,000 samples encompassing purebred and random-bred cats. Over 650,000 polymorphic markers, representing optimal genomic coverage and trait identification, have been selected for a single high-density microarray. Given the relatively low linkage disequilibrium, such a densely populated genotyping array is important for conducting robust complex disease association studies.
Targeted genotyping-by-sequencing (GBS) is essential for identifying SNPs in breeding selection and trait identification. High-throughput targeted GBS applications provide rapid, accurate sequencing results of thousands of markers daily with minimal labor and laboratory inputs.
The AgriSeq ULTRA targeted GBS kit represents the latest advancement in AgriSeq library prep products. With its updated protocol and partial-combinatorial dual barcodes, you can process up to 3,072 uniquely barcoded sample libraries, reducing barcode material to just 4 plates and 8 tubes. This enables sequencing of up to 6,144 samples daily on the Ion GeneStudio S5 System, maximizing your throughput.
Enhancements to the Torrent Suite analysis software and the new AgriSeqVariantCallerLite plugin ensure full data processing in approximately 24 hours or less, significantly reducing wait times between sequencing and results acquisition.
AgriSeq ULTRA is versatile, compatible with plant leaf punches and aquaculture samples like pleopods and fin punches. Our novel AgriSeq Anti-Inhibitor Solution ensures reliable genotype calls from both purified nucleic acids and direct lysates, overcoming PCR inhibitors from crude extractions.
Thermo Fisher Scientific has developed over 300 custom genotyping panels for more than 70 crops using the AgriSeq targeted genotyping by sequencing (T-GBS) workflow on the Ion Torrent platform. We now introduce pre-designed catalog panels for wheat, barley, maize, soybean, strawberry, tomato, pepper, and lettuce. These 300 panels, with various marker densities, are designed for breeding programs, Marker Assisted Selection, Genomic Selection, and 5k SNP performance screening.
Our designs utilize publicly available SNP data and content from the Catalog Axiom microarray platform, based on the latest reference genomes from NCBI. SNPs were selected for minor allele frequency (>5% where applicable), expert breeder input, and public data on population screening.
Each crop panel includes 20-200+ SNP markers for key economic traits such as disease resistance, agronomic traits, crop quality, and yield components. Initial screenings demonstrate these panels' effectiveness for routine breeding material evaluation and custom design optimization. These panels enhance breeding program efficiency through faster, more accurate selection processes.
Purpose: Develop lyophilization-compatible reagents for the RPA (recombinase polymerase amplification) reaction. Develop guidelines for RPA based target detection and NGS library amplification.
Methods: RPA based amplification of DNA/RNA templates, under various conditions and using different detection methods, like end-point detection assay. RPA based library preparation of three bacterial genomes with GC content 33-67%.
Results: Lyo-ready RPA kit; stand-alone RPA proteins and RPA reaction buffer were developed. The kit shows high sensitivity and specificity for both DNA and RNA template amplification, robust amplification from samples containing inhibitors, notable multiplexing capabilities, and compatibility with different detection methods. The RPA proved to be efficient low temperature alternative for NGS library amplification.
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed of 10minutes, increased sensitivity down to 50 copies, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies
For Research Use Only. Not for use in diagnostic procedures.