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FluoroBrite™ DMEM compared to standard phenol red–free DMEM
FluoroBrite™ DMEM has 10% of the background fluorescence emitted by standard phenol red–free DMEM and provides a 9-fold enhancement in signal-to-noise ratio.(A) Images of live epithelial cells that have been labeled with CellLight® Golgi-GFP, BacMam 2.0 (Cat. No. C10592) and cultured in phenol red–free DMEM (Cat. No. 31053) or FluoroBrite™ DMEM with 10% fetal bovine serum. Cells in 10X images are co-labeled with CellLight® Actin-RFP, BacMam 2.0 (Cat. No. C10583). Cells in 40X images (inset) are co-labeled with ER-Tracker™ Red (BODIPY® TR Glibenclamide; Cat. No. E34250). (B) Fluorescence signal from PBS, FluoroBrite™ DMEM, and phenol red–free DMEM at 509 nm in response to excitation at 480 nm. (C) Fluorescent signal from dextran labeled with Alexa Fluor® 488 dye (Cat. No. D22910) over background in PBS, FluoroBrite™ DMEM, and phenol red–free DMEM.
