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Fast, flexible, and spectrally brilliant—simplify high-speed, high-parameter analysis
The Attune Xenith Flow Cytometer is designed to meet the rigorous demands of researchers, leveraging our legacy core acoustic focusing technology. This innovative instrument combines acoustic-assisted hydrodynamic focusing with advanced spectral capabilities, including UV and NIR lasers, supporting both traditional compensation and spectral unmixing analysis.
Experience the power of the Attune Xenith firsthand—request a hands-on demo today!
Enhanced quality and efficiency with acoustic focusing technology
Acoustic-assisted hydrodynamic focusing technology leverages ultrasonic waves to precisely align cells, delivering exceptional speed and flexibility in flow cytometry. This approach enables faster flow rates, analysis of larger cells, and the flexibility to dilute samples. By helping to ensure uniform cell alignment, it provides consistent and accurate measurements essential for high-quality data. The Attune Xenith Flow Cytometer integrates acoustic focusing with advanced spectral flow capabilities, offering outstanding value.
Increase uptime and simplified operation
Experience efficient, high-resolution workflows for spectral and conventional compensation analysis. Now with more colors, faster results, and the ability to evaluate multiple parameters with high sensitivity using minimal sample volume. The Attune Xenith cytometer offers expanded optical capabilities, including UV/NIR, and supports both traditional compensation and spectral unmixing analysis.
Designed with user convenience in mind, new instrument features include:
Extensive detection range for compatibility with large panels
Overview of lasers and fluorescent detectors. The Attune Xenith Flow Cytometer includes 6 lasers (349 nm, 405 nm, 488 nm, 561 nm, 637 nm, and 781 nm) and 51 fluorescent detectors. It also includes 6 scatter detectors for enhanced resolution (488 nm standard FSC and SSC, 405 nm FSC and SSC for small-particle resolution, and 488 nm FSC and SSC for expanded range/polarized detection). The system supports both spectral unmixing and conventional compensation options, making it exceptional for high-parameter workflows.
Application data
Get more information per cell with an easy-to-use spectral analyzer that lets you see more colors
A collection of data showcasing the exceptional performance and capabilities of Attune Xenith Flow Cytometer. This section provides valuable insights and inspiration for your own experiments, allowing you to see data generated using the Attune Xenith Flow Cytometer.
Acoustic focusing allows fast acquisition without compromising resolution
Immunophenotyping of mouse tissue digests using a 32-color spectral unmixing panel. In this study, the advanced acoustic fluidics and high detection sensitivity of the Attune Xenith Flow Cytometer were utilized to conduct broad immunophenotyping, with a specific focus on the development and maturation of B cells across various tissues. The instrument’s clog-resistant design, combined with its acoustic focusing technology, enabled rapid data acquisition without compromising the resolution of the analysis. Even at high cell concentrations of 1 x 107 cells/mL in mouse tissue digests, the Attune Xenith Flow Cytometer demonstrated its capability to deliver accurate and reliable results. To view complete data set view our on-demand seminar, access the seminar.
Rare events without sample concentration; high throughput; exceptional sensitivity for robust data resolution
Precision in rare event detection using a 25-color natural killer cell panel. The study utilized high sensitivity and specificity to interrogate natural killer (NK) cell populations, providing crucial insights for detailed research. Immature, mature and terminal NK populations were identified from human PBMCs, and surface marker expression of each of these subpopulations was characterized. The acquisition of rare events was achieved without the need to concentrate samples, while high sample throughput reduced time to results without compromising population resolution. The sensitivity in detection ensured robust data resolution, enabling the differentiation of even rare populations in high complexity panels. View complete data set here.
Sensitivity in detection facilitates robust data resolution to differentiate even rare populations in high-complexity panels
Exploration of expression profiles of NK cells across activation states. The study utilized the NK cell panel mentioned earlier to investigate the expression of surface markers before and after activation. Human NK cells were incubated with or without IL-2, IL-15, and IL-21 cytokine cocktail for a duration of 48 hours, and the antigen expression was evaluated. The Attune Xenith Flow Cytometer demonstrated strong sensitivity in detecting changes across different stimulation procedures, ensuring reliable and accurate characterization of these rare cell subsets. This highlights the potential of the panel for conducting in-depth analysis and gaining a deeper understanding of NK cell subsets. View complete data set here.
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Dive deeper with our blogs, application notes, and technical notes
Blog articles
Application notes
Technical notes
Publications
Help with dyes and panels
Resources
Here we provide a series of articles to help you get started designing and executing experiments using spectral flow cytometry. Topics include an overview of the differences between conventional and spectral methods, panel design, control and sample preparation, panel evaluation, and data analysis.
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For Research Use Only. Not for use in diagnostic procedures.