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UBE2C Antibody (MA1-173) in WB
Western blot analysis of UBE2C was performed by loading 20ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product ## WT4202). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. UBE2C was detected at ~20kDa using a UBE2C monoclonal antibody (Product ## MA1-173) at a dilution of 1ug/mL in 5% Milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product ## A28177) at a dilution of 1:1,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product ## 34078) and the myECL Imager (Product ## 62236).
