Immunodiagnostic Assay Development with Dynabeads Plus

• Bead surface chemistry optimized for enhanced binding capacity and low non-specific binding
• Freeze dried monosized beads, easy to disperse in buffer before use
• Size: 1 micrometer
• Low sedimentation rate and higher reaction kinetics compared to M-270 beads
• Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules; no further surface activation required

• Immunoassays and immunodiagnostics
• Protein and peptide purification
• Immunoprecipitation

• Hydrophobic bead surface with p-toluene-sulfonyl (tosyl) groups
• Size: 2.8 micrometer, monodisperse
• Iron content (ferrites): 12% (17%)
• Active chemical functionality: 0.1-0.2 mmol/g beads
• Direct covalent binding of proteins/peptides via primary amino- or sulfydryl groups; no further surface activation required

• Immunoassays and immunodiagnostics
• Protein and peptide purification
• Phage display and biopanning
• Cell separation

Dynabeads 
M-280
Streptavidin

• Hydrophobic bead surface
• Based on tosylactivated beads
• Size: 2.8 micrometer, monodisperse
• BSA as blocking protein
• Isoelectric point: pH 5.0
• Low charge (-10 mV, at pH 7)
• Iron content (ferrites): 12% (17%)
• Free biotin binding: 650-900 pmoles/mg beads
• Binding biotinylated Ig: 5-10 µg/mg beads

• Immunoassays and immunodiagnostics
• Isolation of biotinylated molecules
• Protein and peptide purification
• Phage display and biopanning
• Cell isolation

• Hydrophilic bead surface
• Based on carboxylic acid beads
• Size: 2.8 micrometer, monodisperse
• No blocking proteins used
• Isoelectric point: pH 4.5
• Highly charged (-50 mV, at pH 7)
• Iron content (ferrites): 14% (20%)
• Low aggregation of beads in high salt solutions
• Free biotin binding: 650-1,350 pmoles/mg beads
• Binding biotinylated Ig: 5-10 µg/mg beads

• Immunoassays and immunodiagnostics with hydrophobic targets
• Isolation of biotinylated molecules
• Sequence specific DNA/RNA capture in nucleic acid based diagnostics
• For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.

• Hydrophilic bead surface
• Based on carboxylic acid beads (C1)
• Size: 1 micrometer, monodisperse
• No blocking proteins used
• Tween 20 in the buffer
• Isoelectric point: pH 5.2
• Medium charged (-35 mV, at pH 7)
• Iron content (ferrites): 26% (37%)
• Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
• Very low aggregation
• Free biotin binding: > 2,500 pmoles/mg beads
• Binding biotinylated Ig: 20-25 µg/mg beads

• Immunoassays and immunodiagnostics
• Isolation of biotinylated molecules
• Sample preparation of proteins for mass spectrometry
• Sequence specific DNA/RNA capture in nucleic acid based diagnostics