Technical Resources

12000 - MEM alpha, no nucleosides, powder

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.050.00.6666667
L-Alanine89.025.00.28089887
L-Arginine211.0127.00.60189575
L-Asparagine-H2O132.050.00.37878788
L-Aspartic acid133.030.00.22556391
L-Cysteine hydrochloride-H2O176.0100.00.5681818
L-Cystine 2HCl240.024.00.1
L-Glutamic Acid147.075.00.5102041
L-Glutamine146.0292.02.0
L-Histidine209.642.00.20038167
L-Isoleucine131.052.40.4
L-Leucine131.052.40.4
L-Lysine182.772.00.3940887
L-Methionine149.015.00.10067114
L-Phenylalanine165.032.00.19393939
L-Proline115.040.00.3478261
L-Serine105.025.00.23809524
L-Threonine119.048.00.40336135
L-Tryptophan204.010.00.04901961
L-Tyrosine disodium salt261.052.00.19923371
L-Valine117.046.00.3931624
Vitamins
Ascorbic Acid176.050.00.2840909
Biotin244.00.14.0983607E-4
Choline chloride140.01.00.007142857
D-Calcium pantothenate477.01.00.002096436
Folic Acid441.01.00.0022675737
Niacinamide122.01.00.008196721
Pyridoxal hydrochloride204.01.00.004901961
Riboflavin376.00.12.6595744E-4
Thiamine hydrochloride337.01.00.002967359
Vitamin B121355.01.360.0010036901
i-Inositol180.02.00.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.0200.01.8018018
Magnesium Sulfate (MgSO4) (anhyd.)120.097.670.8139166
Potassium Chloride (KCl)75.0400.05.3333335
Sodium Chloride (NaCl)58.06800.0117.24138
Sodium Phosphate monobasic (NaH2PO4-H2O)138.0140.01.0144928
Other Components
D-Glucose (Dextrose)180.01000.05.5555553
Lipoic Acid206.00.29.708738E-4
Phenol Red376.410.00.026567481
Sodium Pyruvate110.0110.01.0

Reference:

1. Simonsen, C.C. and Levinson, A.D. 1983. Isolation and expression of an altered mouse dihydrofolate reductase cDNA. Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499.

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