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Invitrogen™
eBioscience™ 7-AAD Viability Staining Solution
7-AAD (7-amino-actinomycin D) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. 7-AAD can beRead more
| Catalog Number | Quantity |
|---|---|
| 00-6993-50 | 2 mL |
Catalog number 00-6993-50
Price (EUR)
89,25
Each
-
Quantity:
2 mL
Price (EUR)
89,25
Each
7-AAD (7-amino-actinomycin D) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. 7-AAD can be used in place of PI (propidium iodide). This solution can be used in combination with PE (phycoerythrin) and FITC (fluorescein isothiocyanate) conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Reported Application
Flow Cytometric Analysis
Reported Application
Flow Cytometric Analysis
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeCell-Impermeant
Quantity2 mL
ColorRed
Emission535/617
For Use With (Application)Viability Assay
For Use With (Equipment)Flow Cytometer
Product LineeBioscience
Product TypeViability Staining Solution
Unit SizeEach
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Lot #Certificate TypeDateCatalog Number(s)
3161235Certificate of AnalysisJun 12, 202500-6993-50
3051038Certificate of AnalysisMay 23, 202500-6993-50
3165292Certificate of AnalysisMay 15, 202500-6993-50
3052379Certificate of AnalysisMar 21, 202500-6993-50
3051037Certificate of AnalysisFeb 25, 202500-6993-50
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Discrimination between viable and non-viable cells can be carried out with the use of the 7-AAD Viability Dye (Cat. No. 00-6993-50) or Propidium Iodide (PI) Staining Solution (Cat. No. 00-6990-50). The 7-AAD or PI will mark the non-viable cells by binding to the nuclei of those cells. The nucleic acid of viable cells will not be accessible to the dye and will not be stained. When analyzing the data collected, gate out all cells stained with the viability dye. When staining for intracellular proteins, use the Fixable Viability Dye eFluor 455UV, 450, 520, 660, or 780.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.