ProPac™ IMAC-10 HPLC Columns
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Thermo Scientific™

ProPac™ IMAC-10 HPLC Columns

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Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.
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Catalog NumberLength (Metric)Diameter (Metric)Column Format
06327650 mm4 mmAnalytical Column
Catalog number 063276
Price (USD)
929.29
Each
Add to cart
Length (Metric):
50 mm
Diameter (Metric):
4 mm
Column Format:
Analytical Column
Price (USD)
929.29
Each
Add to cart

Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.

Tailored Specificity for IMAC Separation of Proteins
  • Retention control by imidazole concentration or pH gradient
  • Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
  • Effective under native or denaturing HPLC conditions
  • Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
  • Shipped free from any metal and ready to be charged with the metal of your choice.
  • Reusable columns with metal tailored specificity
  • Resolve target proteins using a single column in a high resolution gradient run
  • High purity separations of metal-binding proteins
Includes: 10μm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly(IDA) chains.
Specifications
Column FormatAnalytical Column
Column TypeSeparation of Metal-Binding Proteins
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Green FeaturesResponsibly packaged
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Packing MaterialSpherical, Nonporous Polymer
QuantityEach
Stationary PhaseLysozyne Poly(IDA) Grafts
Temperature60°C
Diameter (Metric)4 mm
Length (Metric)50 mm
Particle Size10 μm
PhaseAffinity Separation
Pore SizeNonporous
Product LineProPac
TechniqueBio-LC
TypeAnalytical Column
pH2 to 12
Unit SizeEach

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