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Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
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| Catalog Number | No. of Reactions |
|---|---|
| 10342053 | 100 Reactions |
| 10342020 | 500 Reactions |
| 10342046 | 1500 Reactions |
| 10342178 | 5000 Reactions |
Catalog number 10342053
Price (EUR)/ Each
85,25
No. of Reactions:
100 Reactions
Price (EUR)/ Each
85,25
Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.
Features
- A choice of recombinant or native enzyme
- Amplification of PCR products up to 5 kb in size
- Enzyme is licensed and qualified for PCR
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- Sequencing ssDNA
- Cycle sequencing
Notes
- One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
- For superior PCR performance, DreamTaq DNA Polymerase is recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration5 U/μL
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatStand-alone enzyme
Hot StartNo
No. of Reactions100 Reactions
Overhang3'-A
PolymeraseTaq DNA Polymerase
Product TypeDNA Polymerase
Quantity100 Units
Reaction FormatSeparate Components
Shipping ConditionDry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
PCR MethodqPCR, Standard PCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• Taq DNA Polymerase (5 U/μL), 20 μL
• 10X PCR buffer, 1.5 mL
• 50 mM MgCl2, 1 mL
Store at -10°C to -30°C in a non-frost-free freezer.
Guaranteed stable for 6 months when stored properly.
• 10X PCR buffer, 1.5 mL
• 50 mM MgCl2, 1 mL
Store at -10°C to -30°C in a non-frost-free freezer.
Guaranteed stable for 6 months when stored properly.