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DMEM/F-12, GlutaMAX™ supplement
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many differentRead more
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| Catalog Number | Quantity |
|---|---|
| 10565042 | 10 x 500 mL |
| 10565018 | 500 mL |
Catalog number 10565042
Price (USD)
523.65
Online Exclusive
570.00Save 46.35 (8%)
Each
Quantity:
10 x 500 mL
Price (USD)
523.65
Online Exclusive
570.00Save 46.35 (8%)
Each
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
| With | Without |
| • GlutaMAX | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineMDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™, GlutaMAX™
Product TypeDMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity10 x 500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Frequently asked questions (FAQs)
Do you offer DMEM/F12 medium without folic acid?
We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
How long can I keep my media after supplementing with serum?
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?
Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?
In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.