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Gibco™
DMEM, high glucose, GlutaMAX™ Supplement, pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
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| Catalog Number | Quantity |
|---|---|
| 10569044 | 10 x 500 mL |
| 10569010 | 500 mL |
| 10569069 | 5 L |
| 10569077 | 10 L |
Catalog number 10569044
Price (USD)
392.65
Online Exclusive
423.00Save 30.35 (7%)
Each
Quantity:
10 x 500 mL
Price (USD)
392.65
Online Exclusive
423.00Save 30.35 (7%)
Each
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
| With | Without |
| • High Glucose | • HEPES |
| • Sodium Pyruvate | |
| • GlutaMAX™ | |
| • Phenol Red |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration1 X
For Use With (Application)Mammalian cell culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™, GlutaMAX™
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity10 x 500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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