Definierte Keratinozyten-SFM (1X)

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Definierte Keratinozyten-SFM (1X)

Q: Are the Defined Keratinocyte SFM basal medium and supplement available separately?

The Defined Keratinocyte SFM basal medium and supplement are not available separately. The kit can be ordered with catalog #10744-019.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center .

Q: Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311. Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center .

Q: Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center .

Q: Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center .

Q: For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees CFind additional tips, troubleshooting help, and resources within our Cell Culture Support Center .

Definiertes Keratinozyten-SFM ist ein serumfreies Medium, das für die Isolierung und Expansion von menschlichen Keratinozyten optimiert wurde, ohne dass RinderhypophysenextraktWeitere Informationen

Katalognummer	Menge
10744019	500 mL

Katalognummer 10744019

Preis (EUR)

240,00

Each

Zum Warenkorb hinzufügen

Menge:

500 mL

Preis (EUR)

240,00

Each

Zum Warenkorb hinzufügen

Definiertes Keratinozyten-SFM ist ein serumfreies Medium, das für die Isolierung und Expansion von menschlichen Keratinozyten optimiert wurde, ohne dass Rinderhypophysenextrakt (BPE) supplementiert oder Fibroblasten-Nährschichten verwendet werden müssen. Die wachstumsfördernde Aktivität von BPE wurde durch die Aufnahme von wachstumsfördernden Wirkstoffen ersetzt, die die konsistente Produktleistung verbessern. Diese wachstumsfördernden Mittel erhalten darüber hinaus die Zellmorphologie, physiologische Marker und das Wachstum. Das Medium ist so formuliert, dass die Fibroblasten-Proliferation gehemmt wird und es eine niedrige Calciumkonzentration (< 0,1 mM) aufweist. Dies ermöglicht die Isolierung und Kultivierung von hochangereicherten Populationen undifferenzierter Keratinozyten.

Definiertes Keratinozyten-SFM ist ein Serum-freies Komplettmedium, bestehend aus definiertem Serum-freiem Keratinozyten-Basalmedium unter Zugabe von definiertem Keratinozyten-SFM Wachstums-Supplement, das die Notwendigkeit von Rinderhypophysenextrakt eliminiert. Das Supplement wird in separater Verpackung mit dem Basalmedium geliefert.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZelltypKeratinozyten, Epithelzellen

Konzentration1 X

ProdukttypKit

Menge500 mL

Haltbarkeit12 Monate

Klassifikationserumfrei

Culture TypeSäugetierzellkultur

FormFlüssig

Serum LevelSerumfrei

SterilitätSteril gefiltert

Sterilization MethodSteril gefiltert

Mit AdditivenGlutamin, Phenolrot

Unit SizeEach

Inhalt und Lagerung

• 500 ml definiertes Keratinozyten-SFM-Basalmedium; vor Licht geschützt im Kühlschrank (2 bis 8 °C) lagern
• 1 ml definiertes Keratinozyten-SFM-Wachstums-Supplement; vor Licht geschützt im Tiefkühlgerät (-5 bis -20 °C) lagern

Komplettmedium: vor Licht geschützt im Kühlschrank (2 bis 8 °C) lagern.

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Scientific Resources

Brochure: Skin and Eye Primary Cell Sourcebook

Serum Free Media

Brochure: Human primary cell culture sourcebook

Brochure: 3D cell culture product selection guide for organoids and spheroids

Brochure: One cell biology—Our cell biology portfolio at a glance

角质细胞培养系统选择指南

Specialty and Serum-Free Media

Altering basal medium components increases the in vitro lifespan of normal human keratinocytes

Keratinocytes

Product Information

Defined Keratinocyte-SFM (1X), liquid

Häufig gestellte Fragen (FAQ)

The Defined Keratinocyte SFM basal medium and supplement are not available separately. The kit can be ordered with catalog #10744-019.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.