KnockOut™ DMEM
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KnockOut™ DMEM
Gibco™

KnockOut™ DMEM

KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells (1). The osmolarityRead more
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Catalog NumberQuantity
10829018500 mL
Catalog number 10829018
Price (USD)
52.75
Each
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Quantity:
500 mL
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Price (USD)
52.75
Each
Add to cart
KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells (1). The osmolarity is optimized to approximate that of mouse embryonic tissue.

Contains no L-glutamine.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeStem Cells (Embryonic), Stem Cells (iPS - Induced Pluripotent Stem)
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product LineKnockOut™
Product TypeKnockOut DMEM
Quantity500 mL
Shipping ConditionRoom Temperature
FormLiquid
With AdditivesHigh Glucose, Phenol Red
Without AdditivesNo Glutamine
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

Can KnockOut SR be used to culture mouse ESCs in feeder-free conditions?

Yes, mouse embryonic stem cells can be cultured using KnockOut SR under feeder-free conditions. Typically, cells are plated at a higher seeding density, in the presence of LIF, on a 0.1% gelatin layer in place of feeder cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can KnockOut SR be used to culture mouse ESCs on feeder cells?

Yes, KnockOut SR was designed to culture mouse ES cells in undifferentiated conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

What are ES cells?

Embryonic stem (ES) cells are derived from the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro while maintaining their potential to differentiate into a wide of range of adult tissues including germ cells. The pluripotency of the ES cells is normally demonstrated in vitro by inducing ES cells to differentiate into embryoid bodies and checking lineage-specific markers for differentiated cells in three body layers (endo, meso, and ectoderm), or injecting them into immunodeficient mice and determining the cell types produced in the teratomas.

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