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Neurobasal™-A Medium
Neurobasal-A Medium is a basal medium designed for long-term maintenance and maturation of pure post-natal and adult brain neurons withoutRead more
| Catalog Number | Quantity |
|---|---|
| 10888022 | 500 mL |
Catalog number 10888022
Price (USD)
112.00
Each
-
Quantity:
500 mL
Price (USD)
112.00
Each
Neurobasal-A Medium is a basal medium designed for long-term maintenance and maturation of pure post-natal and adult brain neurons without the need for an astrocyte feeder layer when used with Gibco B-27 supplements.
Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.
For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.
View publications using Gibco neurobiology products at our Gibco CellCite.
Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.
For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.
View publications using Gibco neurobiology products at our Gibco CellCite.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Cells
Product LineNeurobasal™
Product TypeNeurobasal Medium
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionAmbient
Tested ForEndotoxin, Performance, Sterility, Osmolality, pH
ClassificationSerum-free
Culture TypeNeuronal Cell Culture
FormLiquid
With AdditivesPhenol Red
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.
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Frequently asked questions (FAQs)
No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .
We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.
For more information on Neurobasal media, search "Neurobasal" from our website home page.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Instructions for growing adult neurons can be found in the Neurobasal-A Medium product insert. You can also refer to the following publication for growing adult neurons:
• Isolation and Culture of Adult Rat Hippocampal Neurons by Brewer, G.J., J. Neuroscience Methods 71 (1997), 145-158.
We do not know at what age postnatal neurons require FGF and a higher osmolality medium, or how this could vary based on the species from which the neural tissue is isolated. Without this knowledge, we generally recommend switching to the Neurobasal-A Medium for isolates from animals above 1 week of age. You will need to determine if the Neurobasal-A Medium is optimal for neurons you have from one day old rats.
Further, the addition of bFGF can increase the viability of neurons of any age. Therefore, caution needs to be made in impure neuronal cell culture as bFGF can increase the proliferation of non-neuronal cells. We recommend a pilot experiment to test the impure culture in the presence and absence of bFGF.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We recommend adding B27 and b-FGF to Neurobasal-A Medium to grow adult neurons.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.