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Invitrogen™
Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
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| Catalog Number | No. of Reactions |
|---|---|
| 10966018 | 120 Reactions |
| 10966026 | 300 Reactions |
| 10966034 | 600 Reactions |
| 10966083 | 5000 Reactions |
Catalog number 10966018
Price (USD)
177.65
Online Exclusive
187.00Save 9.35 (5%)
Each
No. of Reactions:
120 Reactions
Price (USD)
177.65
Online Exclusive
187.00Save 9.35 (5%)
Each
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.
The hot-start property allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.
Features
- Convenient reaction assembly at room temperature
- 5' to 3' exonuclease activity
- Lacks 3' to 5' exonuclease activity
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- Hot-start PCR
- RT-PCR
Notes
- For superior PCR performance, the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended.
- Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatTube
Hot StartBuilt-In Hot Start
No. of Reactions120 Reactions
Overhang3'-A
PolymerasePlatinum Taq DNA Polymerase
Product TypeDNA Polymerase
Quantity120 reactions
Reaction FormatSeparate Components
Shipping ConditionWet or Dry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceLow
Reaction SpeedFast or Standard
Unit SizeEach
Contents & Storage
• Platinum Taq DNA Polymerase, 24 μL
• 10X PCR Buffer (without magnesium), 1.25 mL
• 50 mM MgCl2, 1 mL
• KB Extender, 1.25 mL
Store at -10°C to -30°C in a non-frost-free freezer.
• 10X PCR Buffer (without magnesium), 1.25 mL
• 50 mM MgCl2, 1 mL
• KB Extender, 1.25 mL
Store at -10°C to -30°C in a non-frost-free freezer.