Platinum™ GenoType Tsp DNA Polymerase
Platinum&trade; GenoType <i>Tsp</i> DNA Polymerase
Invitrogen™

Platinum™ GenoType Tsp DNA Polymerase

Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.
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Catalog NumberNo. of Reactions
11448024400 Reactions
11448032
also known as 11448-032
2000 Reactions
Catalog number 11448024
Price (USD)
577.65
Online Exclusive
674.00
Save 96.35 (14%)
Each
-
Add to cart
No. of Reactions:
400 Reactions
Price (USD)
577.65
Online Exclusive
674.00
Save 96.35 (14%)
Each
Add to cart
Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic hot-start method that improves PCR specificity and allows for room temperature reaction assembly.

Platinum GenoType Tsp DNA Polymerase features

  • Minimal non-templated nucleotide addition to PCR products
  • Lacks both 5' and 3' exonuclease activity
  • Amplifies fragments up to 500 bp
  • Room temperature reaction assembly

Application

Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.

Unit definition

One unit of Platinum GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min. at 74°C under optimized reaction conditions.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions400 Reactions
OverhangBlunt
PolymeraseTsp Genotyping Polymerase
Quantity250 units
Reaction FormatSeparate Components
Shipping ConditionWet or Dry Ice
Size (Final Product)500 bp or less
Volume50 μL
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• Platinum GenoType Tsp DNA Polymerase (50 μL at 5 Tsp U/μL)
• 10X PCR Buffer (1.25 mL)
• 50 mM MgCl2 (1 mL)

Store at -20°C. Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Is there anything to prevent AmpliTaq Gold DNA polymerase from extending from the 3’ end of a TaqMan probe in a 5’ nuclease assay?

Yes. There is a phosphate group on the 3' end of all TaqMan probes that prevents such extension.

How does AmpliTaq Gold DNA Polymerase differ from AmpliTaq DNA Polymerase?

AmpliTaq Gold DNA Polymerase is a modified form of AmpliTaq DNA Polymerase that contains a proprietary chemical (or so-called hot start molecule) bound to the enzyme's active site. In order to activate the AmpliTaq Gold DNA Polymerase fully, we recommend an initial activation step of 95 degrees C for 10 min when using GeneAmp 10X PCR Buffer I and/or GeneAmp 10X PCR Buffer II and Mg in one of our thermal cyclers. When using GeneAmp 10X PCR Gold Buffer, activation time can be reduced to 5 minutes. Once activation is complete, you can proceed with your standard PCR cycling program (denaturing, annealing, extension, etc).

Does AmpliTaq Gold DNA Polymerase contain exonuclease (proofreading) activity?

No, AmpliTaq Gold DNA polymerase does not contain proofreading activity, however fidelity in PCR amplifications utilizing this enzyme may be improved. High fidelity can be achieved by: 1. Decreasing the final concentration of each nucleotide to 40-50 uM. 2. Using the lowest MgCl2 concentration possible. 3. Using less enzyme. 4. Decreasing extension times. 5. Using the highest annealing temperature possible. 6. Using as few cycles as possible.

Does the fidelity of AmpliTaq DNA Polymerase change in the presence of base analogs?

The fidelity of this PCR enzyme is affected in two ways. First, AmpliTaq DNA Polymerase typically binds to and incorporates base analogs less efficiently than conventional dNTPs, which means that polymerase activity is lower in reactions that contain base analogs. Second, the analog may pair with more than one conventional complementary template base, so the analog may be incorporated at an increased level compared to conventional dNTPs. For the best fidelity, we recommend that base analogs are included at low concentrations in the reaction.

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