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Sf9 cells in Sf-900™ II SFM
Sf9 cells in Sf-900™ II SFM
Gibco™

Sf9 cells in Sf-900™ II SFM

Gibco™ Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco™ Sf9Read more
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Catalog NumberQuantity
114960151.5 mL
Catalog number 11496015
Price (USD)
803.65
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870.00
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1.5 mL
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Price (USD)
803.65
Online Exclusive
870.00
Save 66.35 (8%)
Each
Add to cart
Ask our AI about this Product
Gibco™ Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco™ Sf9 cells are adapted to serum-free suspension culture in Gibco™ Sf-900™ II SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco™ Sf9 cells (frozen in Gibco™ Sf-900™ II SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac™ Baculovirus Expression System, or the InsectDirect™ Expression System.

Small, regular size that generates even monolayers and plaques
Gibco™ Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.

Documented lineage from a low passage Master Cell Bank
Gibco™ Sf9 cells (in Sf-900™ II SFM) were prepared as serum-free, suspension cultures from Sf9 cells that originated at the USDA Insect Pathology Laboratory. The original Sf9 cells were cloned from the parental IPLBSF-21 (Sf21) cell line that was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 34.

Quality and performance testing
Each lot of Gibco™ Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.  This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Media RecommendationSf-900 II SFM (Serum-Free Media)
Product TypeInsect Cells
Quantity1.5 mL
Shipping ConditionDry Ice
Cell LineSf9
Cell TypeInsect Cells
FormLiquid
SpeciesS. frugiperda
Unit SizeEach
Contents & Storage
1 x 1.5 mL (1.0 x 107 cells/mL)

Storage conditions: Liquid nitrogen (vapor phase)

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Frequently asked questions (FAQs)

What is the procedure to thaw frozen insect cells?

The following protocol describes a general procedure for thawing cryopreserved cells. For detailed protocols, always refer to the cell-specific product insert.

1. Remove the cryovial containing the frozen cells from liquid nitrogen storage and immediately place it into a 37°C water bath.
2. Quickly thaw the cells (< 1 minute) by gently swirling the vial in the 37°C water bath until there is just a small bit of ice left in the vial.
3. Transfer the vial into a laminar flow hood. Before opening, wipe the outside of the vial with 70% ethanol.
4. Transfer the desired amount of pre-warmed complete growth medium appropriate for your cell line dropwise into the centrifuge tube containing the thawed cells.
5. Centrifuge the cell suspension at approximately 200 x g for 5-10 minutes. The actual centrifugation speed and duration varies depending on the cell type.
6. After the centrifugation, check the clarity of supernatant and visibility of a complete pellet. Aseptically decant the supernatant without disturbing the cell pellet.
7. Gently resuspend the cells in complete growth medium, and transfer them into the appropriate culture vessel and into the recommended culture environment.

Note: The appropriate flask size depends on the number of cells frozen in the cryovial, and the culture environment varies based on the cell and media type.

Why does the Insect cell line manual state: "Cells should be maintained at 27 degrees C in a non-humidified environment."

Insect cells do not require CO2 or high humidity to grow, they can grow in a lab drawer at room temperature. We recommend this so people don't waste CO2 and other resources necessary for maintaining a tissue culture incubator. It should be noted, however, that the cells will grow in a humidified incubator.

Can I get Sf9 cells that are pre-adapted to Sf-900 II SFM?

In the U.S. we sell Sf9 cells which are adapted to SFM. The catalog number is 11496-015.

When growing Sf9 cells in a bioreactor, can I use a glass vessel that has been cleaned and autoclaved and then reused or do I need to use a disposable vessel?

Yes, you can grow Sf9 cells in glass vessels. The only concern would be if your glass vessels are not clean enough and there may be residual detergent left which will hurt your cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are spindle-shaped Sf9 cells bad? How do I get rid of them?

These cells appear after cultures have been grown for several weeks. These do not seem to be detrimental to plating of high titer stocks or expression. However, if they are in high numbers, it may indicate that the cells are becoming old and that the culture should be re-started with a new stock of frozen cells.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
2986779Certificate of AnalysisNov 01, 202411496015
2817181Certificate of AnalysisFeb 06, 202411496015
2664990Certificate of AnalysisAug 28, 202311496015
2566577Certificate of AnalysisMar 02, 202311496015
2496018Certificate of AnalysisAug 11, 202211496015
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Safety Data Sheets

Limited Use Label Licenses (LULL)

'

Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product and progeny, to perform internal research and development for the sole benefit of the purchaser. The purchase of this product does not grant the purchaser any additional rights, including, without limitation: (a) the right to use the product or its components in commercial applications of any kind, including bioproduction (e.g. use of the product to manufacture therapeutic agents or diagnostic test components, such as proteins, antibodies, viral particles or virus-like particles), quality control and commercial services such as reporting the results of purchaser's activities for a fee or other form of consideration; (b) the right to use the product or its components as a therapeutic agent or diagnostic test component; (c) the right to use the product or its components to produce material for use in human clinical trials; or (d) the right to transfer or resell the product or its components in any form, progeny or derivative. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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Citations & References (11)

Citations & References
Abstract
Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human.
Authors:Rowles J,Scherer SW,Xi T,Majer M,Nickle DC,Rommens JM,Popov KM,Harris RA,Riebow NL,Xia J,Tsui LC,Bogardus C,Prochazka M
Journal:The Journal of biological chemistry
PubMed ID:8798399
Cell cycle-regulated phosphorylation of the Xenopus polo-like kinase Plx1.
Authors: Kelm Olaf; Wind Mathias; Lehmann Wolf D; Nigg Erich A;
Journal:J Biol Chem
PubMed ID:11994303
'Polo-like kinases (Plks) control multiple important events during M phase progression, but little is known about their activation during the cell cycle. The activities of both mammalian Plk1 and Xenopus Plx1 peak during M phase, and this activation has been attributed to phosphorylation. However, no phosphorylation sites have previously been ... More
Variola virus immune evasion design: expression of a highly efficient inhibitor of human complement.
Authors: Rosengard Ariella M; Liu Yu; Nie Zhiping; Jimenez Robert;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12077314
'Variola virus, the most virulent member of the genus Orthopoxvirus, specifically infects humans and has no other animal reservoir. Variola causes the contagious disease smallpox, which has a 30-40% mortality rate. Conversely, the prototype orthopoxvirus, vaccinia, causes no disease in immunocompetent humans and was used in the global eradication of ... More
Alternate interactions define the binding of peptides to the MHC molecule IA(b).
Authors: Liu Xinqi; Dai Shaodong; Crawford Frances; Fruge Rachel; Marrack Philippa; Kappler John;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12084926
'We have solved the crystal structure of the MHCII molecule, IA(b), containing an antigenic variant of the major IA(b)-binding peptide derived from the MHCII IEalpha chain. The four MHC pockets at p1, p4, p6, and p9 that usually bind peptide side chains are largely empty because of alanines in the ... More
Identification and characterization of three members of the human metallocarboxypeptidase gene family.
Authors: Wei Suwen; Segura Sonia; Vendrell Josep; Aviles Francesc X; Lanoue Edith; Day Robert; Feng Yun; Fricker Lloyd D;
Journal:J Biol Chem
PubMed ID:11836249
'Amino acid homology searches of the human genome revealed three members of the metallocarboxypeptidase (metallo-CP) family that had not been described in the literature in addition to the 14 known genes. One of these three, named CPA5, is present in a gene cluster with CPA1, CPA2, and CPA4 on chromosome ... More
11 total citations

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