This kit provides: • Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase • The One-Step SuperScript™ III Reverse Transcriptase for one-step qRT-PCR • SYBR GreenER dye • UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
SYBR
Format
Tube
PCR Method
1-step RT-qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Fast or Standard
For Use With (Equipment)
7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
Product Line
SYBR™ GreenER™
Product Type
One-Step qRT-PCR Kit
Sufficient For
2500 Reactions
No. of Reactions
2500 Reactions
Sample Type
RNA
Contents & Storage
EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX in a separate tube.
Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.