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Gibco™
MEM α, nucleosides, powder
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negativeRead more
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| Catalog Number | Quantity |
|---|---|
| 11900073 | 10 L |
| 11900024 | 10 x 1 L |
Catalog number 11900073
Price (USD)
98.00
Each
In stock
Quantity:
10 L
Price (USD)
98.00
Each
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
| With | Without |
| • Ribonucleosides | • Sodium Bicarbonate |
| • Deoxyribonucleosides | |
| • L-glutamine | |
| • Phenol Red |
The complete formulation is available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMEM α (Minimum Essential Medium α)
Quantity10 L
Shelf Life36 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormPowder
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesLow Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
Without AdditivesNo HEPES, No Sodium Bicarbonate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Media Formulations
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