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MEM (Temin's modification) (2X), no phenol red
Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used ofRead more
| Catalog Number | Quantity |
|---|---|
| 11935046 | 500 mL |
Catalog number 11935046
Price (USD)
78.00
Each
-
Quantity:
500 mL
Price (USD)
78.00
Each
Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Earle's salts | • Phenol Red |
| • Sodium Bicarbonate |
The complete formulation is available.
MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. Temin’s Modification is a 2x MEM with 4x the vitamins and amino acids of the original MEM. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (8.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMEM (Minimum Essential Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, Earle's Balanced Salt Solution (EBSS), Sodium Bicarbonate
Without AdditivesNo HEPES, No Phenol Red, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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