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Catalog Number | Quantity |
---|---|
12309050 | 100 mL |
12309019 | 1000 mL |
OptiPRO™ SFM is a serum-free, animal origin-free culture medium designed for growth of several kidney-derived cell lines including MDBK, MDCK, PK-15 and VERO for virus or recombinant protein production. In addition, OptiPRO™ SFM has successfully grown other cell lines, such as Hela, BHK-21 and COS-7. Gibco™ OptiPRO™ SFM features:
Serum-free, animal origin-free, low protein formulation
Gibco™ OptiPRO™ SFM is formulated with a very low protein concentration (< 10 μg/mL), allowing for easier purification of your recombinant protein.
Direct adaptation for many popular cell lines
Gibco™ OptiPRO™ SFM has been designed for growth of kidney-derived cell lines such as MDCK, MDBK, VERO and PK-15. In addition, OptiPRO™ SFM has successfully grown other cell lines, such as Hela, BHK-21 and COS-7, allowing you to choose a single medium for multiple cell lines.
cGMP Manufacturing and Quality System
Gibco™ OptiPRO™ SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component; (3) reverse engineer the product or cause the product to be reverse engineered; or (4) use the product for purposes other than what is indicated in this Limited Use Label License. Life Technologies is not aware of Intellectual Property ("IP") that would be infringed by the manufacture or sale of its products. Customers are urged to perform an IP search and analysis specific to their manufacturing processes and target biologic products. Should that analysis require information about Life Technologies products, Life Technologies will provide to the customer's IP counsel the information necessary to analyze any relevant IP in a manner that protects Life Technologies proprietary information. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
'For virus titering in OptiPRO SFM we recommend using Falcon or equivalent 96-well plates and plating cells at a concentration of 1 X 10^6/mL. If you are having trouble growing cells for virus titration in OptiPRO SFM in the 96-well plates, we suggest switching to the EMEM with 5%FBS or DMEM with 5% FBS.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The sequential adaptation process is a multi-subculture of cells. We recommend the following passaging sequence:
• First passage: use 25% OptiPRO SFM:75% original stock medium
• Second passage: use 50% OptiPRO SFM:50% original stock medium
• Third passage: use 75% OptiPRO SFM:25% original stock medium
• Fourth and subsequent passages: use 100% OptiPRO SFM
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
You can supplement OptiPRO SFM with 5mL/L Antibiotic-Antimycotic (Cat. No. 15240096)
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We recommend adding L-glutamine or GlutaMAX-I to OptiPRO SFM at a final concentration of 4mM.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
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