OptiPRO™ SFM
OptiPRO™ SFM
Gibco™

OptiPRO™ SFM

OptiPRO™ SFM is a serum-free, animal origin-free culture medium designed for growth of several kidney-derived cell lines including MDBK, MDCK, PK-15 and VERO for virus or recombinant protein production.
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Catalog NumberQuantity
12309050100 mL
123090191000 mL
Catalog number 12309050
Price (EUR)
33,67
Each
Add to cart
Quantity:
100 mL
Customize this product
Price (EUR)
33,67
Each
Add to cart
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OptiPRO™ SFM is a serum-free, animal origin-free culture medium designed for growth of several kidney-derived cell lines including MDBK, MDCK, PK-15 and VERO for virus or recombinant protein production. In addition, OptiPRO™ SFM has successfully grown other cell lines, such as Hela, BHK-21 and COS-7. Gibco™ OptiPRO™ SFM features:

  • Serum-free, animal origin-free, low protein formulation
  • Direct adaptation for many popular cell lines

Serum-free, animal origin-free, low protein formulation

Gibco™ OptiPRO™ SFM is formulated with a very low protein concentration (< 10 μg/mL), allowing for easier purification of your recombinant protein.

Direct adaptation for many popular cell lines

Gibco™ OptiPRO™ SFM has been designed for growth of kidney-derived cell lines such as MDCK, MDBK, VERO and PK-15. In addition, OptiPRO™ SFM has successfully grown other cell lines, such as Hela, BHK-21 and COS-7, allowing you to choose a single medium for multiple cell lines.

cGMP Manufacturing and Quality System

Gibco™ OptiPRO™ SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, MDCK, BHK-21, PK-15, COS-7, MDBK, VERO
Concentration1 X
Endotoxin LevelLow
FormatBottle(s)
Included AntibioticsNo antibiotics
Product LineGibco™
Product TypeSerum Free Medium
Quantity100 mL
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free, Low Protein, Serum-free
Culture TypeAdherent Cell Culture
FormLiquid
Serum LevelSerum-free
With AdditivesPhenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine
Without AdditivesNo Glutamine
Unit SizeEach
Contents & Storage
Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3246156Certificate of AnalysisJun 28, 202512309050, 12309019
3247061Certificate of AnalysisJun 15, 202512309050, 12309019
3118176Certificate of AnalysisJun 15, 202512309050, 12309019
2998691Certificate of AnalysisMay 23, 202512309050, 12309019
3126845Certificate of AnalysisMay 11, 202512309050, 12309019
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Safety Data Sheets

Limited Use Label Licenses (LULL)

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Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component; (3) reverse engineer the product or cause the product to be reverse engineered; or (4) use the product for purposes other than what is indicated in this Limited Use Label License. Life Technologies is not aware of Intellectual Property ("IP") that would be infringed by the manufacture or sale of its products. Customers are urged to perform an IP search and analysis specific to their manufacturing processes and target biologic products. Should that analysis require information about Life Technologies products, Life Technologies will provide to the customer's IP counsel the information necessary to analyze any relevant IP in a manner that protects Life Technologies proprietary information. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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Frequently asked questions (FAQs)

For virus titering in OptiPRO SFM we recommend using Falcon or equivalent 96-well plates and plating cells at a concentration of 1 X 10^6/mL. If you are having trouble growing cells for virus titration in OptiPRO SFM in the 96-well plates, we suggest switching to the EMEM with 5%FBS or DMEM with 5% FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The sequential adaptation process is a multi-subculture of cells. We recommend the following passaging sequence:
• First passage: use 25% OptiPRO SFM:75% original stock medium
• Second passage: use 50% OptiPRO SFM:50% original stock medium
• Third passage: use 75% OptiPRO SFM:25% original stock medium
• Fourth and subsequent passages: use 100% OptiPRO SFM

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

You can supplement OptiPRO SFM with 5mL/L Antibiotic-Antimycotic (Cat. No. 15240096)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend adding L-glutamine or GlutaMAX-I to OptiPRO SFM at a final concentration of 4mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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