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Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
---|---|---|---|---|---|
12337024 | 1,000 reactions | 2,337.65 Online price 2,585.00 | - | ||
12337016 | 200 rxns | 567.65 Online price 590.00 | - |
AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).
Benefits of using AccuPrime™ GC-Rich DNA Polymerase:
• Yield—high yields for targets up to 5 kb in length
• Specificity—AccuPrime™ accessory proteins for improved PCR specificity
• Sensitivity—sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.