CD Hybridoma AGT™ Medium
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Gibco™

CD Hybridoma AGT™ Medium

Gibco™ CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growthRead more
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Catalog NumberQuantity
123720251 L
1237201710 L
12372001
also known as 12372-001
100 L
Catalog number 12372025
Price (USD)
104.65
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110.00
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Quantity:
1 L
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Price (USD)
104.65
Online Exclusive
110.00
Save 5.35 (5%)
Each
Add to cart
Gibco™ CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco™ CD Hybridoma Medium features:
• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin-free, protein-free and chemically defined formulation
• Convenient, easy-to-use Advanced Granulation Technology (AGT™) format

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco™ CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco™ CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin-free, protein-free and chemically defined formulation
Gibco™ CD Hybridoma Medium is animal origin-free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco™ chemically defined media contain no proteins, hydrolysates or components of unknown composition.

Convenient, easy-to-use Advanced Granulation Technology (AGT™) format
Our Advanced Granulation Technology™ (AGT™) format is an easy-to-use dry powder format. The granules dissolve instantly and pH-adjusts for fast media preparation. Complete reconstitution instructions are available in the product manual.

Product Use
For research use/further cell culture manufacturing. Not for use in diagnostic procedures. Customers using Gibco™ CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco™ CD Hybridoma Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMyeloma Cells, Hybridomas
Endotoxin Level≤10 EU/mL, ≤ 10 EU/ml
Product LineAGT™, Gibco™
Product TypeHybridoma Medium
Quantity1 L
Shipping ConditionWet Ice
ClassificationAnimal Origin-free, Chemically-defined, Protein-free
FormPowder (AGT)
Serum LevelSerum-free
SterilityNon-sterile
With AdditivesSodium Pyruvate, Sodium Bicarbonate
Without AdditivesNo Glutamine, No Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C, dark and dry
Shipping conditions: Gel packs
Shelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

What are the main benefits of using AGT?

Complete formulations
- Homogeneous distribution of minute components
- Complete complex formulations in a dry format, including serum-free, protein-free, and chemically defined nutrient media
- Pre-adjusted to the appropriate pH and osmolality
- Require only standard supplementation with L-glutamine for L-glutamine dependent systems
- Can be customized for modified catalog and customers' proprietary formulations

Granular format
- Dissolves instantly for faster media preparation times
- Dust-free granules reduce mess and environmental contamination
- Performance equivalent to liquid media
- Workflow improvement
- Helps reduce total cycle costs due to lower number of components, decreasing time involved in raw material planning, procurement, and testing
- Faster and easier media preparation, with less potential for error
- Easy to scale up from research through production, in batch sizes from 2 kg to 6,000 kg
- Available in various packaging options

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is AGT?

Granulated cell culture media is an easy-to-use, granular form of dry media that is produced by applying fluid bed granulation technology to cell culture nutrient media manufacturing. The patented process allows production of complete formulations of a variety of serum-free, protein-free, and chemically defined media in a dry format.

AGT offers the benefits of liquid media without the cost, storage, and transportation issues. It is a complete medium that is pH and osmolality pre-adjusted. The granules dissolve instantly for faster media preparation time than conventional dry powder media. AGT media can therefore help reduce total cycle costs, decreasing time involved in raw material planning, procurement, and testing as well as media preparation. These benefits are all realized while maintaining comparable cell growth to liquid media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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