POROS™ 50 HQ Strong Anion Exchange Resin

Certificates

SDS

Thermo Scientific™

POROS™ 50 HQ Strong Anion Exchange Resin

Thermo Scientific™ POROS™ Strong Anion Exchange Media. POROS 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing.

Change view

Quantity:

250 mL

1 L

5 L

10 L

Catalog number 1255907

also known as 1-2559-07

Price (USD)

3,660.00

Each

Add to cart

Quantity:

1 L

Price (USD)

3,660.00

Each

Add to cart

POROS 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing. POROS HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.

POROS High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.

Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
Chemical stability: Allows aggressive cleaning and sanitization
Enhanced biomolecule access: Provided via large pores, ranging between 500-10,000 Å
Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed

In contrast to conventional chromatography media, POROS High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large 'throughpores' allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short 'diffusive' pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1000 to 5000 cm/hr compared to 50 to 360 cm/hr for conventional media.

Specifications

Pore Size500 to 10,000 Å

Product LinePOROS™

Recommended StorageRoom Temperature

Shipping ConditionRoom Temperature

Stationary PhaseHQ

Quantity1 L

Unit SizeEach

What are the benefits of POROS chromatography resins in a manufacturing process?

How many uses should I expect out of my POROS or CaptureSelect column?

Can POROS resins be used to purify large biomolecules?

What type of column do you recommend for POROS 50 µm resins? Do I need a high pressure column for POROS resins?

This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all POROS™ 50 HQ Strong Anion Exchange Resin FAQs