TrypLE™ Select Enzyme (1X), no phenol red

Gibco™

TrypLE™ Select Enzyme (1X), no phenol red

Gibco™ TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.

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Catalog Number	Quantity
12563011	100 mL
12563029	500 mL
12563030	5 L
12563031	10 L

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Catalog number 12563011

Price (USD)

52.50

Each

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Quantity:

100 mL

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Delivery information

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Price (USD)

52.50

Each

Add to cart

Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, TrypLE Select is:

Gentle on cells—maintains cell health for reproducible results
Room-temperature stable—convenient to use
Easy to use—substitutes directly into existing protocols with no inactivation required
Animal origin free (AOF)—pure, consistent, and effective

This TrypLE Select is modified as follows:

With

EDTA

Without

Phenol Red

Gentle on cells

TrypLE Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable

TrypLE Select is room-temperature stable and ready to use. TrypLE Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use

TrypLE Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)

Unlike porcine trypsin and other enzymes, TrypLE Select is free of animal-derived components and is formulated on dedicated animal origin-free equipment.

Product Intended Use

Customers using TrypLE Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System

TrypLE Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

Specifications

Cell TypeMammalian Cells

ChelatorsEDTA

Concentration1 X

Culture TypeAdherent Cell Culture

Endotoxin LevelLow

FormatBottle(s)

Green FeaturesEnergy efficient, Sustainable packaging

Osmolality280 - 300 mOsm/kg

Product LineGibco™

Quantity100 mL

RemarksRoom Temperature Stable

Shelf Life24 Months

Shipping ConditionRoom Temperature

SourceAnimal origin-free

Tested ForIn vitro Bioassay

ClassificationAnimal Origin-free

FormLiquid

Product TypeCell Culture Dissociation Reagent

Without AdditivesNo Phenol Red

pH7 to 7.4

Unit SizeEach

Contents & Storage

Storage conditions: 15–30°C, protect from light.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.

Media Formulations

TrypLE™ Select (1X), no phenol red

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Frequently asked questions (FAQs)

Can I use TrypLE Select Enzyme to detach cells from glass surfaces?

Yes, TrypLE Select Enzyme can detach cells from glass surfaces.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the stability of TrypLE Select Enzyme (1X), no phenol red, (Cat. Nos. 12563029, 12563011) at 15-30 degrees C, 2-8 degrees C, and -5 to -20 degrees C?

The shelf life of TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563029, 12563011) is 24 months from the date of the manufacturer when stored at 15-30 degrees C, or 2-8 degrees C, and protected from light.

It can be stored at -5 to -20 degrees C for at least 22 months without affecting its performance.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563029, 12563011) sensitive to light?

We do not have specific data on the light sensitivity of TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563029, 12563011). However, the protease generated by the confidential organism has been observed to be less effective when exposed to constant light. Therefore, we recommend storing the product away from light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can you provide a statement regarding the customer’s responsibility to ensure the elimination of a reagent, such as the TrypLE Select Enzyme, from their drug product and determination of drug product safety?

The following statement is regarding the customer’s responsibility to ensure the elimination of a reagent, such as the TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563011, 12563029), from their drug product and determination of drug product safety:

"Products are not manufactured by Thermo Fisher under a pharmaceutical quality management system to excipient requirements and are not intended to be used as excipients. Customers are responsible for properly qualifying any products it intends to use in or as excipients – this may include steps such as washing, filtration, and sterilization of these products."

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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