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B-27™ Supplement (50X), minus vitamin A
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B-27™ Supplement (50X), minus vitamin A
Gibco™

B-27™ Supplement (50X), minus vitamin A

A variant of the original serum-free B-27 Supplement, designed for the proliferation of neural stem cells in the absence of vitamin A.
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Catalog NumberQuantity
1258701010 mL
12587001100 mL
Catalog number 12587010
Price (USD)
170.65
Online Exclusive
184.00
Save 13.35 (7%)
Each
Add to cart
Quantity:
10 mL
Price (USD)
170.65
Online Exclusive
184.00
Save 13.35 (7%)
Each
Add to cart

Gibco B-27 Supplement minus vitamin A is a customized, serum-free formulation based on the original, most cited neuronal cell culture supplement, designed specifically to support the proliferation and cultivation of neural stem cells (NSCs) and neural progenitor cells. By excluding vitamin A (also known as retinyl acetate or retinol), this variant prevents the differentiation-inducing effects of retinoic acid, making it ideal for maintaining NSCs and neural progenitors in their undifferentiated state.

  • Optimized for NSC proliferation—by removing vitamin A, this supplement is perfectly suited for the growth and expansion of embryonic and adult neural stem cells and neural precursors, without inducing differentiation
  • Versatile cultivation—supports the cultivation of neural progenitor cells and NSCs in both neurospheres in suspension and adherent monolayer cultures, providing flexibility in your experimental design. Ideal for cell culture where retinoic acid signaling must be controlled.
  • High quality—cGMP-manufactured to ensure the highest standards of quality and consistency. Each batch undergoes rigorous performance testing, including assessments of its ability to support the differentiation of human neural stem cells to neurons.
  • Comprehensive testing—every lot is tested for endotoxins, sterility, and mycoplasma to ensure the utmost safety and reliability
  • Proven performance—derived from the proprietary formulation of the original B-27 Supplement, trusted by researchers worldwide for its consistent and reliable results

Choose Gibco B-27 Supplement, minus vitamin A, for an optimized, high-quality solution that supports the proliferation of neural stem cells while preventing unwanted differentiation influenced by retinoic acid, ensuring consistent and reliable results for your research.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Progenitor Cells
Concentration50 X
Culture TypeMammalian Cell Culture
Product LineB-27™
Quantity10 mL
Serum LevelSerum-Free
Shelf Life12 Months
Shipping ConditionDry Ice
SourceSerum-Free
Tested ForEndotoxin, Performance, Sterility, Mycoplasma
ClassificationSerum-free
FormLiquid
Product TypeCell Culture Supplement
SterilitySterile
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -20°C) and protect from light.
Media Formulations
B-27 Supplement Minus Vitamin A (50X) liquid

Frequently asked questions (FAQs)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all B-27™ Supplement (50X), minus vitamin A FAQs

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