Sf9 cells in Sf-900™ III SFM
Product Image
Gibco™

Sf9 cells in Sf-900™ III SFM

Gibco™ Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco™ Sf9Read more
Have Questions?
Catalog NumberQuantity
126590171 Vial
Catalog number 12659017
Price (USD)
813.65
Online Exclusive
870.00
Save 56.35 (6%)
Each
In stock
Add to cart
Quantity:
1 Vial
Price (USD)
813.65
Online Exclusive
870.00
Save 56.35 (6%)
Each
Add to cart
Gibco™ Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco™ Sf9 cells are adapted to serum-free suspension culture in Gibco™ Sf-900™ III SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco™ Sf9 cells (frozen in Gibco™ Sf-900™ III SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac™ Baculovirus Expression System, or the InsectDirect™ Expression System.

Good growth in adherent or suspension culture
Each vial containing 1 × 107 cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco™ Sf-900™ III SFM are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.

Small, regular size that generates even monolayers and plaques
Gibco™ Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.

Documented lineage from a low passage Master Cell Bank
Gibco™ Sf9 cells (in Sf-900™ III SFM) were prepared as serum-free, suspension cultures from Sf9 cells that originated at the USDA Insect Pathology Laboratory. The original Sf9 cells were cloned from the parental IPLBSF-21 (Sf21) cell line that was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 26.

Quality and performance testing
Each lot of Gibco™ Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Media RecommendationSf-900 III SFM (Serum-Free Media)
Product LineSf-900™
Product TypeInsect Cells
Quantity1 Vial
Shipping ConditionDry Ice
Cell LineSf9
Cell TypeInsect Cells
SpeciesS. frugiperda
Unit SizeEach
Contents & Storage
1 x 1.5 mL (1 x 107 cells/mL)
Storage conditions: liquid nitrogen (vapor phase)
Shipping conditions: frozen

Frequently asked questions (FAQs)

What is the procedure to thaw frozen insect cells?

The following protocol describes a general procedure for thawing cryopreserved cells. For detailed protocols, always refer to the cell-specific product insert.

1. Remove the cryovial containing the frozen cells from liquid nitrogen storage and immediately place it into a 37°C water bath.
2. Quickly thaw the cells (< 1 minute) by gently swirling the vial in the 37°C water bath until there is just a small bit of ice left in the vial.
3. Transfer the vial into a laminar flow hood. Before opening, wipe the outside of the vial with 70% ethanol.
4. Transfer the desired amount of pre-warmed complete growth medium appropriate for your cell line dropwise into the centrifuge tube containing the thawed cells.
5. Centrifuge the cell suspension at approximately 200 x g for 5-10 minutes. The actual centrifugation speed and duration varies depending on the cell type.
6. After the centrifugation, check the clarity of supernatant and visibility of a complete pellet. Aseptically decant the supernatant without disturbing the cell pellet.
7. Gently resuspend the cells in complete growth medium, and transfer them into the appropriate culture vessel and into the recommended culture environment.

Note: The appropriate flask size depends on the number of cells frozen in the cryovial, and the culture environment varies based on the cell and media type.

Why does the Insect cell line manual state: "Cells should be maintained at 27 degrees C in a non-humidified environment."

Insect cells do not require CO2 or high humidity to grow, they can grow in a lab drawer at room temperature. We recommend this so people don't waste CO2 and other resources necessary for maintaining a tissue culture incubator. It should be noted, however, that the cells will grow in a humidified incubator.

Can Sf9 cells in Sf-900 III SFM (Cat. No. 12659017) be thawed and grown in Sf-900 II SFM instead?

It should be okay to thaw the cells into Sf-900 II SFM. This is a richer media compared to the Sf-900 III SFM so the cells would have an easy time adapting. We would recommend taking the cells through 3 passages in the new medium before using them for any experiments as that they have enough time to adapt.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When growing Sf9 cells in a bioreactor, can I use a glass vessel that has been cleaned and autoclaved and then reused or do I need to use a disposable vessel?

Yes, you can grow Sf9 cells in glass vessels. The only concern would be if your glass vessels are not clean enough and there may be residual detergent left which will hurt your cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are spindle-shaped Sf9 cells bad? How do I get rid of them?

These cells appear after cultures have been grown for several weeks. These do not seem to be detrimental to plating of high titer stocks or expression. However, if they are in high numbers, it may indicate that the cells are becoming old and that the culture should be re-started with a new stock of frozen cells.

Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
2886925Certificate of AnalysisJul 25, 202412659017
2580697Certificate of AnalysisApr 11, 202312659017
2480637Certificate of AnalysisAug 26, 202212659017
2363189ACertificate of AnalysisAug 03, 202112659017
2363189Certificate of AnalysisAug 03, 202112659017
5 results displayed, search above for a specific certificate

Safety Data Sheets

Limited Use Label Licenses (LULL)

'

Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product and progeny, to perform internal research and development for the sole benefit of the purchaser. The purchase of this product does not grant the purchaser any additional rights, including, without limitation: (a) the right to use the product or its components in commercial applications of any kind, including bioproduction (e.g. use of the product to manufacture therapeutic agents or diagnostic test components, such as proteins, antibodies, viral particles or virus-like particles), quality control and commercial services such as reporting the results of purchaser's activities for a fee or other form of consideration; (b) the right to use the product or its components as a therapeutic agent or diagnostic test component; (c) the right to use the product or its components to produce material for use in human clinical trials; or (d) the right to transfer or resell the product or its components in any form, progeny or derivative. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

'

Share catalog number, name or link