KnockOut™ DMEM/F-12
Taking your work to the clinic? Check out CTS KnockOut DMEM/F-12 »
KnockOut™ DMEM/F-12
Gibco™

KnockOut™ DMEM/F-12

Low osmolality medium without L-glutamine or HEPES buffer optimized for the growth of human embryonic and induced pluripotent stem cells.Read more
Have Questions?
Catalog NumberQuantity
12660012500 mL
Catalog number 12660012
Price (USD)
67.00
Each
Add to cart
Quantity:
500 mL
Customize this product
Price (USD)
67.00
Each
Add to cart
Low osmolality medium without L-glutamine or HEPES buffer optimized for the growth of human embryonic and induced pluripotent stem cells.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeStem Cells (Embryonic), Stem Cells (iPS - Induced Pluripotent Stem)
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product LineGibco™
Product TypeKnockOut DMEM/F-12
Quantity500 mL
Shipping ConditionRoom Temperature
FormLiquid
With AdditivesPhenol Red
Without AdditivesNo Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Store at 2° to 8°C and protect from light.

Frequently asked questions (FAQs)

What is the concentration of glucose in KnockOut DMEM/F-12 Medium?

KnockOut DMEM/F-12 Medium contains 3151.0 mg/L of glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does KnockOut DMEM/F-12 contain Knockout Serum Replacement (KSR) supplement?

KnockOut DMEM/F-12 does not contain Knockout Serum Replacement (KSR) supplement; it would need to be added prior to use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link