Nunc™ Polycarbonate Cell Culture Inserts in Multi-Well Plates
Save 51% on plasticware essentials and stock your lab to culture with confidence. View promotion details >
Share
Nunc™ Polycarbonate Cell Culture Inserts in Multi-Well Plates
Thermo Scientific™

Nunc™ Polycarbonate Cell Culture Inserts in Multi-Well Plates

Cultivate most cell types without matrix coating when using Thermo Scientific™ Nunc™ Polycarbonate Membrane Inserts in Multidishes.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberPore SizeDish TypeCulture Area
1406200.4 μm24-Well Plate0.47 cm2
1406273 μm24-Well Plate0.47 cm2
1406298 μm24-Well Plate0.47 cm2
1406400.4 μm6-Well Plate3.14 cm2
1406423 μm6-Well Plate3.14 cm2
1406448 μm6-Well Plate3.14 cm2
1406520.4 μm12-Well Plate1.13 cm2
1406543 μm12-Well Plate1.13 cm2
1406568 μm12-Well Plate1.13 cm2
1406600.4 μm6-Well Plate4.1 cm2
1406633 μm6-Well Plate4.1 cm2
1406688 μm6-Well Plate4.1 cm2
Catalog number 140620
Price (USD)
510.00
Case of 48
In stock
Add to cart
Pore Size:
0.4 μm
Dish Type:
24-Well Plate
Culture Area:
0.47 cm2
Price (USD)
510.00
Case of 48
Add to cart
Cultivate most cell types without matrix coating when using Thermo Scientific™ Nunc™ Polycarbonate Membrane Inserts in Multidishes.
Features:
  • Tissue culture-treated porous membrane for optimized cell attachment
  • Three pore sizes: 0.4, 3, and 8 micron
  • Pre-packed in sterile Nunclon™ Delta-treated multi-well plates
  • Nontoxic and nonpyrogenic

Applications:

  • Tumor migration and invasion assays
  • Epithelial (e.g. Caco-2) and endothelial (e.g. brain microvascular endothelial cells) barriers assays
  • Dermal/epidermal and epithelial tissue models (e.g. artificial skin model)
  • Chemotaxis studies
  • Co-culture

Warranty: 90 days

 

WARNING: Cancer – www.P65Warnings.ca.gov
Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

General References:

  1. Mardi A Crane-Godreau et.al (2005). CCL20/macrophage Inflammatory Protein 3alpha and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment With Lipopolysaccharide or Pam3Cys. PMID: 15618187
  2. Rettinger CL et. al (2018). Quantitative Assessment of Retina Explant Viability in a Porcine Ex Vivo Neuroretina Model. PMID: 29924674
  3. Mardi A Crane-Godreau et.al (2004). Effect of Escherichia coli and Lactobacillus rhamnosus on macrophage inflammatory protein 3 alpha, tumor necrosis factor alpha, and transforming growth factor beta release by polarized rat uterine epithelial cells in culture. PMID: 15039305
  4. Soboll G et. al (2006). Effect of oestradiol on PAMP-mediated CCL20/MIP-3 alpha production by mouse uterine epithelial cells in culture. PMID: 16771853
  5. Mardi A Crane-Godreau et. al (2009). Exposure to Cigarette Smoke Disrupts CCL20-Mediated Antimicrobial Activity in Respiratory Epithelial Cells. PMID: 19966927
  6. Wildemann B et. al (2005). Differences in the fusion and resorption activity of human osteoclasts after stimulation with different growth factors released from a polylactide carrier. PMID: 15477999
  7. Sugita Y et. al (1997). Pigment epithelium-derived factor (PEDF) has direct on the metabolism and proliferation of microglia and indirect effects on astrocytes. PMID: 9335258
  8. van Schilfgaarde M et. al (1999). Haemophilus influenzae localized in epithelial cell layers is shielded from antibiotics and antibody-mediated bactericidal activity. PMID: 10222210
  9. Chung HC et. al (2014). Inhibition of porcine endogenous retrovirus in PK15 cell line by efficient multitargeting RNA interference. PMID: 24138389
  10. Ode A et. al (2010). Toward biomimetic materials in bone regeneration: functional behavior of mesenchymal stem cells on a broad spectrum of extracellular matrix components. PMID: 20878902
Specifications
ColorClear
DescriptionPolycarbonate Cell Culture Insert with 0.4 micron pore size in 24-well plate
Dish Type24-Well Plate
No. per Case48
No. per Pack12
Surface TreatmentCell culture-treated
Culture Area0.47 cm2
For Use WithMultidish 24
Inserts per Plate12
MaterialPolycarbonate (Membrane)
No. of Wells24
Pore Density<0.85 x 108 pores/cm2
Pore Size0.4 μm
SterilitySterile
TypeCulture Insert
Volume (Metric) Working0.5 mL
Unit SizeCase of 48

Frequently asked questions (FAQs)

What is the difference between the 24-well plate format for the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620) and the 24-well plate format for the Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002)?

The difference between the two 24-well plate formats is as follows:

  • Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620): There are only 12 inserts in the 24-well multi-well plate, leaving every second column free without an insert. This is a preferred configuration for some co-culture experiments, as it makes it easy to access the culture grown in the multi-well plate for media changes, etc., by placing the insert in the empty well next to it. The inserts are placed directly into the wells here, leaving a 0.9 mm gap between the bottom of the well and the insert membrane.
  • Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002): There are 24 inserts in the 24-well carrier plate. The carrier plate with the hanging slots allows for adjustment of the insert height. The three hanging positions in the carrier plate allow for distance of approximately 0.9 mm, 3.3 mm, or 6.3 mm between the well-bottom and insert growth surface for both 12- and 24-well plate formats. This configuration is preferred when customer needs more versatility, for example to facilitate air-liquid interface culture, and other co-culture applications.


Note: details on plate configuration can also be found within the following product brochure (pg 3).

With the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates, what is the distance between the cell culture membrane and the bottom of the multi-well dish?

The membrane is approximately 1.0 mm above the bottom of the dish for the Nunc Polycarbonate Cell Culture Inserts.

Can I observe cells on the cell culture membrane when the Nunc Polycarbonate Cell Culture Insert is inside of a multi-well plate?

Due to the large pore size of the Nunc polycarbonate inserts, spots or holes are observed and they represent the membrane. In this plane of focus, the most visible cells are the ones located on the membrane, whereas the slightly blurred backdrop are the cells on the dish that are in a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of the membrane. However, these cells are visible in culture.

What is the recommended Multidish chamber media volume per carrier plate insert and height position?

Low position: 2 mL (12-well), 1 mL (24-well), Medium position: 3 mL (12-well), 1.5 mL (24-well), High position: 4 mL (12-well), 2 mL (24-well). Some optimization may be necessary for development purposes.

Why are only 12 inserts provided with standard membrane insert products in the 24-well format?

This has emerged as the industry standard. In many cases having empty wells facilitates media changes and other experimental steps.

View all Nunc™ Polycarbonate Cell Culture Inserts in Multi-Well Plates FAQs

Documents & Downloads

Certificates

Search by lot number or partial lot number
Search
Request a Certificate

Safety Data Sheets

Share catalog number, name or link