EBSS, no calcium, no magnesium, no phenol red

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Gibco™

EBSS, no calcium, no magnesium, no phenol red

Earle's Balanced Salt Solution (EBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more

Catalog Number	Quantity
14155063	500 mL

Catalog number 14155063

Price (USD)

43.09

Each

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Quantity:

500 mL

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Price (USD)

43.09

Each

Add to cart

Earle's Balanced Salt Solution (EBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Gibco™ EBSS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.

This EBSS is manufactured as follows:

With	Without
• Glucose	• Calcium
• Sodium Bicarbonate	• Magnesium
	• Phenol Red

The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

AdditiveNo Sodium Pyruvate

ClassificationAnimal Origin-Free

Dilution1 X

For Use With (Application)Variety of Cell Culture Applications; Such as Washing Cells Before Dissociation; Transporting Cells or Tissue Samples; Diluting Cells for Counting; and Preparing Reagents

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Osmolality270 - 310 mOsm/kg

Phenol Red IndicatorNo Phenol Red

Product LineGibco™

Quantity500 mL

Shipping ConditionRoom Temperature

SterilitySterile-filtered

FormLiquid

Product TypeEarle's Balanced Salt

Solution TypeEarle's Balanced Salt

pH7.0 - 8.1

Unit SizeEach

Contents & Storage

Store at room temperature.

Shelf life: 36 months from date of manufacture

Media Formulations

14155 - EBSS, no calcium, no magnesium, no phenol red

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Frequently asked questions (FAQs)

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

EBSS, no calcium, no magnesium, no phenol red

Frequently asked questions (FAQs)

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Do you offer media without phenol red (phenol red-free media)?

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Safety Data Sheets