HBSS, no calcium, no magnesium, no phenol red

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HBSS, no calcium, no magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more

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Catalog Number	Quantity
14175095	500 mL
14175103	10 x 500 mL
14175079	1000 mL
14175145	6 x 1000 mL

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Catalog number 14175095

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31.65

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Quantity:

500 mL

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Price (USD)

31.65

Online Exclusive

33.13

Save 1.48 (4%)

Each

Add to cart

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.

This HBSS is modified as follows:

With	Without
• Glucose	• Calcium
	• Magnesium
	• Phenol Red

The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

AdditiveNo Sodium Pyruvate

ColorClear

Dilution1 X

For Use With (Application)Mammalian Cell Culture

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Osmolality270 - 295 mOsm/kg

Phenol Red IndicatorNo Phenol Red

Product LineGibco™

Quantity500 mL

Shipping ConditionRoom Temperature

SterilitySterile-filtered

FormLiquid

Product TypeHanks' Balanced Salt Solution (HBSS)

Solution TypeHank's Balanced Salt

pH6.7 - 7.8

Unit SizeEach

Contents & Storage

Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 36 months from date of manufacture

Explore all Gibco Balanced Salt Solutions to enable increased culture stability ›

Media Formulations

14175 - HBSS, no calcium, no magnesium, no phenol red

Fast, reliable, hassle-free cell counting with Countess 3

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Frequently asked questions (FAQs)

Do you offer a 10X version of the HBSS, no calcium, no magnesium, no phenol red (Cat. No. 14175095)?

Yes, we do offer HBSS (10X), no calcium, no magnesium, no phenol red (Cat. No. 14185052).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

HBSS, no calcium, no magnesium, no phenol red

Frequently asked questions (FAQs)

Do you offer a 10X version of the HBSS, no calcium, no magnesium, no phenol red (Cat. No. 14175095)?

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Do you offer media without phenol red (phenol red-free media)?

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