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DPBS (10X), no calcium, no magnesium
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DPBS (10X), no calcium, no magnesium
Gibco™

DPBS (10X), no calcium, no magnesium

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washingRead more
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Catalog NumberQuantity
14200075Promo Image500 mL
14200166Promo Image10 x 500 mL
Catalog number 14200075
Price (USD)
60.65
Online Exclusive
66.00
Save 5.35 (8%)
Each
Add to cart
Quantity:
500 mL
Customize this product
Price (USD)
60.65
Online Exclusive
66.00
Save 5.35 (8%)
Each
Add to cart

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ DPBS require pH adjustment and filtration at the time of preparation (see protocol for details).

We offer a variety of Gibco™ DPBS formulations for a range of cell culture applications.


This DPBS is modified as follows:
Without
• Calcium
• Magnesium
• Phenol Red


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
ColorClear
Dilution10 X
For Use With (Application)Mammalian Cell Culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality2630 - 3000 mOsm/kg
Phenol Red IndicatorNo Phenol Red
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Product TypeDPBS
Solution TypeDulbecco's Phosphate Buffered Saline
pH6.7 - 7.0
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 36 months from date of manufacture

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Frequently asked questions (FAQs)

Do you offer an alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538)?

We do not have a direct alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538). However, we do have alternative primary and secondary antibodies, as well as reagents, that can be used for trilineage differentiation. Please note that we have not internally validated the use of all these reagents together.

We recommend the following primary antibodies:

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)) (Cat. No. MA5-11547)

alpha-Fetoprotein Monoclonal Antibody (AFP3) (Cat. No. 14-6583-80)

beta-3 Tubulin Monoclonal Antibody (2G10) (Cat. No. MA1-118)

We recommend the following secondary antibodies:

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 555 (Cat. No. A21137)

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 594 (Cat. No. A21135)

https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG1-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21121

We recommend using the following reagents:

https://www.thermofisher.com/order/catalog/product/88-8824-00

NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (Cat. No. R37606)

Blocker BSA (Cat. No. 37520)

DPBS (10X), no calcium, no magnesium (Cat. No. 14200075)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I get a specific value for the conductivity for your DPBS products?

Sorry, we do not test the conductivity of our DPBS products and hence cannot provide a value or range.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your DPBS products DNase/RNase-free? Can I use them for RNA work?

Our DPBS products are for cell culture use and have not been tested for DNase/RNase activity. We do not recommend using these products for RNA work.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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