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Citations & References (22)
Gibco™
Antibiotic-Antimycotic (100X)
Solution contains penicillin, streptomycin, and Gibco Amphotericin B to prevent cell culture contamination.
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| Catalog Number | Quantity |
|---|---|
| 15240062 | 100 mL |
| 15240096 | 20 mL |
| 15240112 | 20 x 100 mL |
Catalog number 15240062
Price (USD)
54.65
Online Exclusive
57.50Save 2.85 (5%)
Each
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Quantity:
100 mL
Price (USD)
54.65
Online Exclusive
57.50Save 2.85 (5%)
Each
Gibco™ Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 μg/mL of streptomycin, and 25 μg/mL of Gibco Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Amphotericin B prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast.
Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Amphotericin B is an antifungal agent that prevents the growth of fungi and yeast by causing an increase in fungal plasma membrane permeability.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 X
Culture TypeMammalian Cell Culture
Quantity100 mL
Shelf Life12 Months
Shipping ConditionDry Ice
FormLiquid
Product TypeAntibiotic-Antimycotic
SterilitySterile-filtered
Sterilization MethodSterile-filtered
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture
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Lot #Certificate TypeDateCatalog Number(s)
2779741Certificate of AnalysisJan 29, 202515240096, 15240062
2585887Certificate of AnalysisJan 29, 202515240096, 15240062
2585852Certificate of AnalysisJan 29, 202515240096, 15240062
2585853Certificate of AnalysisJan 29, 202515240096, 15240062
2585889Certificate of AnalysisJan 29, 202515240096, 15240062
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SDSScientific Resources
Frequently asked questions (FAQs)
The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The components are in a 0.85% saline solution.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of shipment.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (22)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Common variation at 6q16 within HACE1 and LIN28B influences susceptibility to neuroblastoma.
Journal:Nat Genet
PubMed ID:22941191
Neuroblastoma is a cancer of the sympathetic nervous system that accounts for approximately 10% of all pediatric oncology deaths. Here, we report a genome-wide association study of 2,817 neuroblastoma cases and 7,473 controls. We identified two new associations at 6q16, the first within HACE1 (rs4336470; combined P=2.7×10(-11); odds ratio 1.26,
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells)
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic âlivers in a dish.â We describe strategies to induce
Bombyxin is a growth factor for wing imaginal disks in Lepidoptera.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12429853
'The mechanisms that control the growth rate of internal tissues during postembryonic development are poorly understood. In insects, the growth rate of imaginal disks varies with nutrition and keeps pace with variation in somatic growth. We describe here a mechanism by which the growth of wing imaginal disks is controlled.
22 total citations