Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30SRead more
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15750078
10 x 10 mL
15750060
10 mL
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Catalog number 15750078
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666,00
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10 x 10 mL
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Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used to prevent the contamination of cell cultures by bacteria. The recommended working concentration ranges from 0.5 to 50 μg /mL.
Dual-Site cGMP Manufacturing Gibco™ Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ Gentamicin product made in our Scotland facility (15750-037). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration50 mg/mL
Recommended StorageStorage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture
Shipping ConditionRoom Temperature
Sterile FilteredYes
Validated ApplicationPrevention of Cell Culture Contamination
Physical FormLiquid
Quantity10 x 10 mL
TypeGentamicin
Unit SizeEach
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Frequently asked questions (FAQs)
How can I decontaminate my cultures?
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
What antibiotics do you offer to help control or eliminate cell culture contamination?
Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).