Platinum™ Taq DNA Polymerase, DNA-free
Platinum™ Taq DNA Polymerase, DNA-free
Invitrogen™

Platinum™ Taq DNA Polymerase, DNA-free

Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.
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Catalog NumberNo. of Reactions
15966005400 Reactions
159660252000 Reactions
Catalog number 15966005
Price (EUR)
358,00
Each
Add to cart
No. of Reactions:
400 Reactions
Request bulk or custom format
Price (EUR)
358,00
Each
Add to cart
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the high sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.

Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.

Features

  • Certified low DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
  • Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
  • Same performance as Platinum Taq DNA Polymerase manufactured using conventional process

Applications

  • Amplification of DNA from complex genomic and viral templates
  • Hot start PCR
  • RT-PCR
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration5 U/μL
Fidelity (vs. Taq)1X
FormatTube
Hot StartBuilt-In Hot Start
No. of Reactions400 Reactions
Overhang3'-A
PolymerasePlatinum Taq DNA Polymerase
Product TypeTaq DNA Polymerase
Quantity500 Units
Reaction FormatSeparate Components
Shipping ConditionDry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• Platinum Taq DNA Polymerase, DNA-free, 100 μL
• 10X PCR Buffer (without magnesium), DNA-free, 1.25 mL
• 50 mM MgCl2, DNA-free (1 mL)

Store at -20°C in a non-frost-free freezer.

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