Embryonic Stem Cell FBS, qualified, US origin

Gibco™

Embryonic Stem Cell FBS, qualified, US origin

Q: What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies. Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol 1 . 1. Pellerin, et al., Bioengineering, published in 2021.Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center .

Q: Do I need to heat-inactivate Embryonic Stem-Cell FBS, qualified?

The material is not heat-inactivated. We would only recommend heat-inactivating it if your application specifically requires it. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center .

Fetal Bovine Serum, ES cell qualified, is specially tested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.

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Catalog Number	Quantity	Format
16141002	50 mL	One Shot
16141079	500 mL	Bottle

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Catalog number 16141002

Price (USD)

148.00

Each

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Quantity:

50 mL

Format:

One Shot

Price (USD)

148.00

Each

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Fetal Bovine Serum, ES cell qualified, is specially tested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells. It is Gibco FBS quality and performance you trust offered in a single-use FBS aliquot, which provides greater convenience, saves time, and is easy to use.

Sera Category: Specialty

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

Specifications

Donor HerdNo

Endotoxin Concentration<50 EU/mL

Hemoglobin Concentration≤25 mg/dL

Product LineOne Shot™

Purity or Quality GradeES Cell-Qualified

Shipping ConditionFrozen

SpeciesCattle/Bovine

Tested ForBovine Respiratory Syncytial Virus FA, Cytopathic Agents, Hemadsorbing Agents, Reovirus FA, Rabies FA, Hemoglobin (ng/dL), Blue Tongue Virus, Bovine Adenovirus FA, Bovine Parvovirus FA, Osmolality, Sp2 Performance Assay, Electrophoretic Pattern, Total Protein, Endotoxin (EU/mL), Mycoplasma, Sterility Testing (Bacteria and Fungi)

AgeFetal

Country of OriginUnited States

FormLiquid

FormatOne Shot

Product TypeFetal Bovine Serum

Quantity50 mL

Serum TreatmentStandard (Sterile-filtered)

SterilitySterile

Unit SizeEach

Contents & Storage

Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Do I need to heat-inactivate Embryonic Stem-Cell FBS, qualified?

The material is not heat-inactivated. We would only recommend heat-inactivating it if your application specifically requires it. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Embryonic Stem Cell FBS, qualified, US origin

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Do I need to heat-inactivate Embryonic Stem-Cell FBS, qualified?

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