The Thermo Scientific pCMV-
Gaussia Luc vector is a derivative of the pMCS-
Gaussia Luc vector containing the
Gaussia luciferase gene under the control of the CMV (Cytomegalovirus) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pCMV-
Gaussia Luc vector:
• Strong (CMV) constitutive promoters for co-transfection and normalization
• Naturally-secreting
Gaussia luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
The
Gaussia Luc Vectors contain a gene cloned from the copepod,
Gaussia princeps. The gene encodes a naturally-secreted bioluminescent
Gaussia luciferase (approx. 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.
These vectors are subject to a limited use label license.
More Product Data•
Highly sensitive multiplex luciferase reporter assays•
Luciferase assays in hard-to-transfect Jurkat cells•
Monitoring neuronal differentiation using multiplexed luciferase reporters•
Activation of the antioxidant response pathway by pesticide chemicalsRelated ProductspMCS-Gaussia Luc Vector for Luciferase AssayspTK-Gaussia Luc Vector for Luciferase AssaysFor Research Use Only. Not for use in diagnostic procedures.