FreeStyle™ MAX Reagent
FreeStyle™ MAX Reagent
Gibco™

FreeStyle™ MAX Reagent

FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily beRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
1644750015 mL
164471001 mL
1644775010 x 15 mL
Catalog number 16447500
Price (USD)
10,344.65
Online Exclusive
10,900.00
Save 555.35 (5%)
Each
Add to cart
Quantity:
15 mL
Price (USD)
10,344.65
Online Exclusive
10,900.00
Save 555.35 (5%)
Each
Add to cart
FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins. FreeStyle™ MAX Reagent allows the highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications, and is specifically formulated for use with:

• FreeStyle™ 293-F Cells (suspension human embryonal kidney cells, Cat. No. R790-07) in serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018)
• FreeStyle™ CHO-S Cells (suspension Chinese Hamster Ovary cells, Cat. No. R800-07) in serum-free FreeStyle™ CHO Expression Medium (Cat. No. 12651-014)
• DG44 Cells (DHFR suspension CHO cells) in CD DG44 Medium with 8 mM L-glutamine and 18 mL/L 10% Pluronic™ F-68 (Cat. No. A11000-01)

Product use
FreeStyle™ MAX Reagent is intended for use with the FreeStyle™ MAX 293 Expression System (Cat. No. K9000-10) and the FreeStyle™ MAX CHO Expression System (Cat. No. K9000-20).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ClassificationAnimal Origin-Free
For Use With (Application)Transfection
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LineFreestyle™
Product TypeTransfection Reagent
Quantity15 mL
Serum CompatibleYes
Shipping ConditionWet Ice
Cell TypeEstablished Cell Lines
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Each unit contains one vial of reagent. Store at +4°C. FreeStyle™ MAX is guaranteed stable for 6 months when stored properly.

Frequently asked questions (FAQs)

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can FreeStyle MAX reagent be used for transfecting adherent cells?

Yes, the reagent can be used for adherent cells, however the protocol supplied with the reagent has been optimized for CHO and 293 cells in suspension culture. If you want to transfect adherent cells, please use Lipofectamine 2000 transfection reagent.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link

1x1 image pixel for data collection