UltraPure™ Low Melting Point Agarose
UltraPure™ Low Melting Point Agarose
Invitrogen™

UltraPure™ Low Melting Point Agarose

Green features
UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications.Read more
Catalog NumberQuantity
1652005050 g
16520100100 g
Catalog number 16520050
Price (JPY)
78,700
Each
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Quantity:
50 g
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UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. Benefits of using UltraPure™ Low Melting Point Agarose:

• Ideal for analysis and recovery of DNA and RNA for routine applications
• Strong gel structure allows for better handling and less breakage
• Ideal for a variety of applications such as in-gel manipulation, tissue and cell culture, and viral plaque assays
• Can also be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay)

Improved packaging
The new environmentally friendly packaging uses 75% less plastic than the original bottles (see figure). This means less energy and raw material used in manufacture, and less waste in landfills. Additionally, the easy-pour spout reduces the likelihood of spills and contamination.

Performance and quality testing
The performance of UltraPure™ Agarose is evaluated to satisfy specifications in appearance, moisture, gel strength, gelling temperature, melting temperature, sulfate content, electroendosmosis, and DNase/RNase assay.

Note: UltraPure™ Low Melting Point Agarose (Cat. No. 16520-100) is a replacement for the item previously sold under Cat. No. 15517-022.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Nucleic Acid Gel Electrophoresis, Protein Electrophoresis, Blotting
FormPowder
Gel CompatibilityAgarose Gels
Green FeaturesSustainable packaging
Melting PointLow Melting Point
Product LineUltraPure™
Product TypeAgarose
Quantity50 g
Shipping ConditionRoom Temperature
Separation Range100 to >30000 bp
Unit SizeEach
Contents & Storage
UltraPure™ Agarose products are packaged in new eco-friendly pouches that use less material than conventional plastic bottles. Store at 15° to 30°C.
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
0001385636Certificate of AnalysisJun 26, 202516520100
0001389642Certificate of AnalysisJun 26, 202516520050
0001383982Certificate of AnalysisJun 26, 202516520100
0001385634Certificate of AnalysisJun 04, 202516520050
0001381477Certificate of AnalysisJun 04, 202516520050
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Safety Data Sheets

Frequently asked questions (FAQs)

Our UltraPure Agarose is standard melting-point agarose designed for routine separation and analysis of DNA and RNA fragments in the 500-23,000 nt range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. We also offer an UltraPure Low Melting Point Agarose, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a low melting temperature of 65°C or less.

Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Citations & References (6)

Citations & References
Abstract
Coenzyme Q10 protects Pc12 cells from cisplatin-induced DNA damage and neurotoxicity.
Authors:da Silva Machado C, Mendonça LM, Venancio Vde P, Bianchi ML, Antunes LM
Journal:Neurotoxicology
PubMed ID:23434506
'The purpose of this study was to investigate the neuroprotective effect of a water-soluble formulation of coenzyme Q10 (WS-CoQ10) in PC12 cells exposed to cisplatin, a chemotherapeutic agent with a dose-limiting factor due to neurotoxicity. In the cytokinesis-block micronucleus cytome assay (CBMN Cyt), WS-CoQ10 (at concentrations of 0.1, 0.5 and ... More
Spontaneous glycine-induced calcium transients in spinal cord progenitors promote neurogenesis.
Authors:Brustein E, Côté S, Ghislain J, Drapeau P
Journal:Dev Neurobiol
PubMed ID:22888055
Glycine and GABA are depolarizing during early development, but the purpose of this paradoxical chloride-mediated depolarization remains unclear, especially at early stages. It was previously reported that suppressing glycine signaling from the beginning of development in zebrafish embryos caused an abnormal maintenance of the progenitor population and a specific reduction ... More
Cytotoxicity and DNA fragmentation properties of butylated hydroxyanisole.
Authors:Vandghanooni S, Forouharmehr A, Eskandani M, Barzegari A, Kafil V, Kashanian S, Ezzati Nazhad Dolatabadi J
Journal:DNA Cell Biol
PubMed ID:23413972
Butylated hydroxyanisole (BHA) is a synthetic phenolic food additive that is used to prevent oils, fats, and shortenings from oxidative deterioration and rancidity. It was tested for potential cytotoxicity and genotoxicity upon A549 lung cancer cells. MTT assay and flow cytometry analysis were used for cytotoxicity assessment, whereas genotoxicity was ... More
A short cis-acting motif in the M112-113 promoter region is essential for IE3 to activate M112-113 gene expression and is important for murine cytomegalovirus replication.
Authors:Perez KJ, Martínez FP, Cosme-Cruz R, Perez-Crespo NM, Tang Q
Journal:J Virol
PubMed ID:23255797
Immediate-early 3 (IE3) gene products are required to activate early (E)-stage gene expression of murine cytomegaloviruses (MCMV). The first early gene activated by IE3 is the M112-113 gene (also called E1), although a complete understanding of the activation mechanism is still lacking. In this paper, we identify a 10-bp cis-regulating ... More
A mutant prion protein sensitizes neurons to glutamate-induced excitotoxicity.
Authors:Biasini E, Unterberger U, Solomon IH, Massignan T, Senatore A, Bian H, Voigtlaender T, Bowman FP, Bonetto V, Chiesa R, Luebke J, Toselli P, Harris DA
Journal:J Neurosci
PubMed ID:23392670
Growing evidence suggests that a physiological activity of the cellular prion protein (PrP(C)) plays a crucial role in several neurodegenerative disorders, including prion and Alzheimer's diseases. However, how the functional activity of PrP(C) is subverted to deliver neurotoxic signals remains uncertain. Transgenic (Tg) mice expressing PrP with a deletion of ... More
6 total citations

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