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Thermo Scientific™
Nunc™ Lab-Tek™ Chamber Slide System
Grow anchorage dependent cells right on the microscope slide using the unique removable chamber of the Thermo Scientific™ Nunc™ Lab-Tek™ Chamber Slide System.
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| Catalog Number | Wells | Material | Unit Size |
|---|---|---|---|
| 177410 | 1 | Permanox Plastic | Case of 96 |
| 177372 | 1 | Soda Lime Glass | Case of 96 |
| 177372PK | 1 | Soda Lime Glass | Pack of 16 |
| 177380 | 2 | Soda Lime Glass | Case of 96 |
| 177380PK | 2 | Soda Lime Glass | Pack of 16 |
| 177399 | 4 | Soda Lime Glass | Case of 96 |
| 177399PK | 4 | Soda Lime Glass | Pack of 16 |
| 177402 | 8 | Soda Lime Glass | Case of 96 |
| 177402PK | 8 | Soda Lime Glass | Pack of 16 |
| 177410PK also known as 177410 | 1 | Permanox Plastic | Pack of 16 |
| 177429 | 2 | Permanox Plastic | Case of 96 |
| 177429PK also known as 177429 | 2 | Permanox Plastic | Pack of 16 |
| 177437 | 4 | Permanox Plastic | Case of 96 |
| 177437PK | 4 | Permanox Plastic | Pack of 16 |
| 177445 | 8 | Permanox Plastic | Case of 96 |
| 177445PK | 8 | Permanox Plastic | Pack of 16 |
| 178599 | 16 | Soda Lime Glass | Case of 96 |
| 178599PK | 16 | Soda Lime Glass | Pack of 16 |
Catalog number 177410
Price (USD)
1,354.65
Online Exclusive
1,394.00Save 39.35 (3%)
Case of 96
-
Wells:
1
Material:
Permanox Plastic
Unit Size:
Case of 96
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Price (USD)
1,354.65
Online Exclusive
1,394.00Save 39.35 (3%)
Case of 96
Grow, stain, and examine cells directly on the Thermo Scientific™ Nunc™ Lab-Tek™ Chamber Slide™, which consists of a polystyrene media chamber attached to a microscopic slide. Slide surface is specially treated for adherent cell culture. Choose either specialty washed Soda Lime Glass slide or cell attachment optimized Permanox Plastic slide. Media chamber is easily removable without the need for a tool.
Features:
- The solvent-resistant Permanox™ Plastic Chamber Slides are available in 1-, 2-, 4- and 8-well formats.
- The specialty washed Soda Lime Glass Chamber Slides are available in 1-, 2-, 4-, 8- and 16-well formats.
- Media chambers can be removed from slides without the need for a tool
- Medical-grade non-toxic silicone sealing gasket can be removed with a pair of pointy tweezers before applying coverslip.
- "Sand blasted" writing area provides easy identification of samples.
- Coverglass 171080 is to be used with the 16-well glass Chamber Slide 178599.
Ordering Information:
- Can be purchased in case of 96 or pack of 16.
- Shipped sterile in convenient stackable incubation trays.
- Warranty: 90 days
WARNING: Cancer – www.P65Warnings.ca.gov
Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
Specifications
Closure ColorClear
Description1-well Chamber Slide w/ removable well
IncludesClear polystyrene lid with handle on the side for aseptic manipulations
Lid MaterialPolystyrene
No. per Pack16
Culture Area9.4 cm2
Dimensions (LxW)75 x 25 mm
MaterialPermanox Plastic
SterilitySterile
Surface TreatmentNunclon Delta
Volume (Metric) Working2.5 to 4.5 mL
Wells1
Unit SizeCase of 96
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Frequently asked questions (FAQs)
Unfortunately, we do not have a recommended cell density for our LabTek Chamber Slides. However, you can refer to the following link, which provides recommended cell densities for our other cell culture products:
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/cell-culture-useful-numbers.html
Please review the following key differences between the Nunc Lab-Tek Chamber Slide System and the Nunc Lab-Tek II Chamber Slide System:
- The Nunc Lab-Tek Chamber Slide System is available in Permanox plastic or glass and incorporates a silicone gasket.
- The Nunc Lab-Tek II Chamber Slide System is available in glass only and do not have a silicone gasket.
- The Nunc Lab-Tek II Chamber Slide System has a blue hydrophobic border and the upper structure is attached by an adhesive. Supplied with this product is a key and holder system which is used to remove the upper structure from the glass slide.
- The Nunc Lab-Tek Chamber Slide System is available in 1-, 2-, 4-, 8- or 16-well formats.
- The Nunc Lab-Tek II Chamber Slide System is available in 1-, 2-, 4- or 8-well formats.
The following are possible causes and solutions:
- The cells could be stressed due to media substitution, especially from serum-free formulations, or other changes to the culture environment such as in temperature, humidity, and CO2 percentage. If possible, always plate cells in serum-containing medium. Switching cells to a serum-free media should be done gradually.
- The cells could be plated at very low densities. Most cell lines have a minimal platting density below which normal cell proliferation cannot occur, and therefore should be avoided.
- The cells could be poorly adherent. Treatment of cells that may reduce adhesion and therefore, should not be done immediately after plating, but after the cells have resumed exponential growth.
The upper structure of our Nunc Lab-Tek Chamber Slide products are made of polystyrene so it is not compatible with many fixation reagents. These chamber slide products are designed for upper structure and gasket removal before fixing, staining, and coverslipping.
Our Nunc Lab-Tek Chamber Slide products are used for fluorescence analyses at the microscopic level.
The following are commonly used excitation and emittance wavelengths:
FITC
- Excitation: 420-490 nm
- Emittance: 520-550 nm
Rhodamine
- Excitation: 510-560 nm
- Emittance: 570-610 nm
Hoechst
- Excitation: 330-380 nm
- Emittance: 480-510 nm