Ethidium Bromide Solution (0.625 mg/mL)
Ethidium Bromide Solution (0.625 mg/mL)
Thermo Scientific™

Ethidium Bromide Solution (0.625 mg/mL)

Thermo Scientific Pierce Ethidium Bromide is available as a dilute (0.625 mg/mL) solution to accommodate most molecular biology applications forRead more
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Catalog NumberQuantity
178965 mL
Catalog number 17896
Price (USD)
100.00
Each
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Quantity:
5 mL
Price (USD)
100.00
Each
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Thermo Scientific Pierce Ethidium Bromide is available as a dilute (0.625 mg/mL) solution to accommodate most molecular biology applications for fluorescent DNA visualization.

Features of Ethidium Bromide Solution:

• Each drop contains 25 μg of ethidium bromide and is ideal for a 50 mL agarose gel
• After adding one drop of solution per 50 mL of gel, the final concentration of EtBr will be 0.5 μg/mL, which is the recommended concentration for electrophoresis of nucleic acids
• Can also be used as the running buffer or for staining gels after electrophoresis

Related Products
Ethidium Bromide Solution (10 mg/mL)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodFluorescence
Product TypeEthidium Bromide Solution
Quantity5 mL
Target MoleculeRNA, DNA
Label or DyeEthidium bromide
Unit SizeEach
Contents & Storage
Store at room temperature, protected from light.
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Frequently asked questions (FAQs)

Do you have a protocol for ethidium bromide staining in agarose gels?

For use in agarose gels:

- Add ethidium bromide to melted agarose to a final concentration of 0.5 µg/mL. Do not melt agarose that already contains ethidium bromide.

For staining agarose gels after electrophoresis:

- You can stain gels that have been run in the absence of ethidium bromide by covering the gel in 0.5 µg/mL ethidium bromide in water and gently agitating for 10 to 30 minutes.
- If necessary, gels can be destained by shaking in H2O for an additional 30 minutes. To stain RNA gels, you should minimize staining time and definitely include a destaining period.

What can ethidium bromide be used to detect and what is its sensitivity?

Ethidium bromide can be used to detect ssDNA, RNA, and dsDNA. This fluorescent dye intercalates between the stacked bases of nucleic acids, and exhibits an increased fluorescence at 590 nm. Ethidium bromide can detect down to ~1-10 ng/band of dsDNA in an agarose gel.

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